Field experiment was conducted to analyze the prospects of two isolated bacteria Bacillus subtilis, Pseudomonas florescence, and one fungal isolate Trichoderma harzianum to evaluate corm rot of Gladiolus grandiflorus L. caused by pathogenic fungus Fusarium oxysporum. Experiment was arranged in RCBD with 5 replicates. Suspensions of these agents individually and in the combination of two and three were applied to the plants at the seedling stage. All treatments provide significant results against disease incidence severity in the field. These bio control agents restrain the disease in stem and rots. B. subtilis showed more significant result against the disease severity % and number of lesion corms-1. B. subtilis treatment significantly enhanced the root and shoot length of Gladiolus plant. The present experiment revealed that corm rot of Gladiolus grandiflorus L. can be efficiently managed by using bio controls agents.


Gladiolus grandiflorus L., Biocontrol agents, Fusarium oxysporum, Corm rot.


Hashmi, I.H., Aslam, A., Farooq, T.H., Zaynab, M., Munir, N., Tayyab, M., Abbasi, K.Y., 2018. Antifungal Activity of Biocontrol Agents against Corm Rot of Gladiolus grandiflorus L. Caused by Fusarium oxysporum. Int. J. Mol. Microbiol., 1(1): 29-37.