The antimicrobial potential of some solvent extracts of Withania somnifera leaves against different strains of bacteria and fungi was investigated. The leaves of W. somnifera species were collected from Bacha Khan University, Charsadda, Pakistan, rinsed with distilled water and extracted with chloroform and ethanol. Antibacterial activity of crude extracts of W. somnifera was determined by agar well diffusion method against gram positive, gram negative bacterial and antifungal activity was determined by agar diffusion method against fungal strains. All tested plant extracts showed varying zones of inhibition against bacteria and fungi tested. The highest zone of inhibition of 27 mm diameter was noted in S. aureus with ethanol extract while the lowest 7 mm zone of inhibition was reported in Klebsiella pneumonia. Among the fungal species, promising level of antifungal activity was observed in all fractions against A. flavus and A. niger. The results obtained exhibit that this plant has good medicinal potential, and it needs further phytochemical exploitation to isolate phytochemical constituents having antibacterial and antifungal activities.


Antibacterial, Antifungal, activity, W. Somnifera, leaves, extracts.


Shuaib, M., Ali, S., Ali, K., Hussain, F., Ilyas, M., Arif, M., Hussain, F., 2019. Validation of the Ethnopharmacological Uses of Withania somnifera. Int. J. Nanotechnol. Allied Sci., 3(1): 1-6.