Validation of the Ethnopharmacological Uses of Withania somnifera
Corresponding Author: Muhammad Shuaib Email: firstname.lastname@example.org
Article Type: Research Article Published: Jan. 31, 2019 Pages: 1-6
DOI: Views 512 Downloads0
The antimicrobial potential of some solvent extracts of Withania somnifera leaves against different strains of bacteria and fungi was investigated. The leaves of W. somnifera species were collected from Bacha Khan University, Charsadda, Pakistan, rinsed with distilled water and extracted with chloroform and ethanol. Antibacterial activity of crude extracts of W. somnifera was determined by agar well diffusion method against gram positive, gram negative bacterial and antifungal activity was determined by agar diffusion method against fungal strains. All tested plant extracts showed varying zones of inhibition against bacteria and fungi tested. The highest zone of inhibition of 27 mm diameter was noted in S. aureus with ethanol extract while the lowest 7 mm zone of inhibition was reported in Klebsiella pneumonia. Among the fungal species, promising level of antifungal activity was observed in all fractions against A. flavus and A. niger. The results obtained exhibit that this plant has good medicinal potential, and it needs further phytochemical exploitation to isolate phytochemical constituents having antibacterial and antifungal activities.
Antibacterial, Antifungal, activity, W. Somnifera, leaves, extracts.
Shuaib, M., Ali, S., Ali, K., Hussain, F., Ilyas, M., Arif, M., Hussain, F., 2019. Validation of the Ethnopharmacological Uses of Withania somnifera. Int. J. Nanotechnol. Allied Sci., 3(1): 1-6.
Abou-Douh, A.M., 2002. New Withanolides and Other Constituents from the Fruit of Withania somnifera. Architec. Pharmacol., 335: 267-276.
Agarwal, R., Prasad, J., 1999. Are individual differences germane to the acceptance of new information technologies? Decision Sci., 30(2): 361-391.
Alavijeh, P.K., Alavijeh, P.K., Sharma, D., 2012. A study of antimicrobial activity of few medicinal herbs. Asian J. Plant Sci. Res., 2(4): 496-502.
Ali, K., Shuaib, M., Ilyas, M., Hussain, F., Hussain, F., 2017. Medicinal Uses of Chemical Extracts from Withania somnifera and Its Antimicrobial Activity: A Mini-Review. PSM. Microbiol., 2(1): 20-23.
Ali, K., Shuaib, M., Hussain, Z., Sajjad, W., Ali, F., Fazil, M., 2016. Ethnobotanical assessment of the medicinal flora of Khyber Agency, Pakistan. Pak. J. Weed Sci. Res., 22(4): 607-616.
Archana, R., Namasivayam, A., 1998. Ant stress or effect of Withania somnifera. J. Ethnopharmacol., 64(1): 91-93.
Bahl, S.R., Del Alamo, J.A., 1993. A new drain-current injection technique for the measurement of off-state breakdown voltage in FETs. IEEE T. Electron. Dev., 40(8): 1558-1560.
Bonjar, S., 2004. Evaluation of Antibacterial Properties of Some Medicinal Plants Used in Iran. J. Ethnopharmacol., 94(2-3): 301-305.
Carron, E.A., Maran, J.M., Montero, L., Fernandozalgo, A., Dominguez, A., 1987. Antimicrobial properties of some extracts obtained from some Mediterranean plants of medicinal value. Plantes Medicinales et Phytotherapie., 21:195-202.
Chang, Q., Khare, G., Dani, V., Nelson, S., Jaenisch, R., 2006. The disease progression of Mecp2 mutant mice is affected by the level of BDNF expression. Neuron., 49(3): 341-348.
Choudhary, M. I., Parveen, Z., Jabbar, A., Ali, I., 1995. Antifungal steroidal lactones from Withania coagulance. Phytochem., 40(4): 1243-1246.
Das, B.N., Saha, A., Ahmed, M., 2009. Anti-inflammatory activity of bark of Xeromphis spinosa. Bangladesh J. Pharmacol., 4(1):76-78.
Hussain, F., Kalim, M., Ali, H., Ali, T., Khan, M., Xiao, S., Iqbal, M.N., Ashraf, A., 2016. Antibacterial Activities of Methanolic Extracts of Datura inoxia. PSM Microbiol., 01(1): 33-35.
Iqbal, M.N., Anjum, A.A., Ali, M.A., Hussain, F., Ali, S., Muhammad, A., Irfan, M., Ahmad, A., Shabbir, A., 2015. Assessment of Microbial Load of Un-pasteurized Fruit Juices and in vitro Antibacterial Potential of Honey against Bacterial Isolates. The Open Microbiol. J., 9: 26-32. DOI: 10.2174/1874285801509010026
Iqbal, M.N., Ali, S., Anjum, A.A., Muhammad, K., Ali, M.A., Wang, S., Khan, W.A., Khan, I., Muhammad, A., Mahmood, A., Irfan, M., Ahmad, A., Ashraf, A., Hussain, F., 2016. Microbiological Risk Assessment of Packed Fruit Juices and Antibacterial Activity of Preservatives against Bacterial Isolates. Pak. J. Zool., 48(6): 1695-1703.
Kalim, M., Hussain, F., Ali, H., Iqbal, M.N., 2016. Antifungal activities of Methanolic Extracts of Datura inoxia. PSM Biol. Res., 01(2): 70-73.
Khodaei, M., Jafari, M., Noori, M., 2012. Remedial use of withanolides from Withania coagolans (Stocks) Dunal. Adv. Life Sci., 2(1):6-19.
Mothana, R.A., Lindequist, U., 2005. Antimicrobial activity of some medicinal plants of the island Soqotra. J. Ethnopharmacol., 96(1): 177-181.
Nisar, M., Kaleem, W.A., Qayum, M., Marwat, I.K., Zia-ulhaq, M., Ali, I., Choudhary, M.I., 2011. Biological screening of zizyphus Oxyphylla edgew stem. Pak. J. Bot., 43(1): 311-317.
Parihaar, R.S., Bargali, K., Bargali, S.S., 2014. Diversity and uses of ethnomedicinal plants associated with traditional agroforestry systems in Kumaun Himalaya. Indian J. Agric. Sci., 84(12): 1470-1476.
Rai, M., Acharya, D., Sing, A., Varma, A., 2001. Positive growth responses of the medicinal plants Spilanthes calvaand Withania somnifera to inoculation by Piriformospora indica in a field trial. Mycorrhiza., 11(3): 123-128.
Rasool, M., Varalakshmi, P., 2006. Immuno-modulatory role of Withania somnifera root powder on experimental induced inflammation: An in vivo and in-vitro study. Vascular Pharmacol., 44(6):406-410.
Scartezzini, P., Speroni, E., 2000. Review on some plants of Indian traditional medicine with antioxidant activity. J. Ethnopharmacol. 71(1): 23-43.
Shahzad, M.I., Ashraf, H., Iqbal, M.N., Khanum, A., 2017. Medicinal Evaluation of Common Plants against Mouth Microflora. PSM Microbiol., 2(2): 34-40.
Sharma, S., Grandvaux, N., Zhou, G.P., Lin, R., Hiscott, J., 2003. Triggering the interferon antiviral response through an IKK-related pathway. Sci., 300(5622): 1148-1151.
Sharma, B.K., Rai, S.K., Rai, D.R., Choudhury, D.R., 2004. Prevalence of intestinal parasitic infestation in school children in the northeastern part of Kathmandu Valley, Nepal. Southeast Asian J. Trop. Med. Public Health., 35(3): 501-505.
Sumbul, S., Ahmad, M.A., Asif, M., Akhtar, M., 2011. Myrtus communis Linn. A review. Indian J. Nat. Prod. Resour., 2(4): 395-402.
Uddin, Q., Samiulla, L., Singh, V.K., Jamil, S.S., 2012. Phytochemical and Pharmacological Profile of Withania somnifera Dunal: A Review. J. App. Pharmac. Sci., 02(01): 170-175.
Get Article Updates