Prevalence of Hepatitis B Surface Antigen among Orphans Children Living in Orphanage in Sana’a City, Yemen

Author's: Amal Mohammed Banafa1, Wadhah Hassan Edrees1,2*, Ghada Hassein Al-Falahi3, Wadee Abdullah Al-Shehari4
Authors' Affiliations
1Medical Laboratory Department, Faculty of Medical Sciences, Al-Razi University, Yemen.2Medical Microbiology Department, Faculty of Applied Sciences, Hajjah University, Yemen.3Medical Laboratory Department, Faculty of Medical Sciences, Sana'a University, Yemen.4Department of Microbiology, Faculty of Medical Sciences, Ibb University, Yemen.
*CorrespondenceWadhah Hassan;
Article Type: Research Article     Published: Mar. 17, 2022 Pages: 19-26
DOI:        Views 243       Downloads 0


Hepatitis B virus (HBV) is considered a serious public health issue in Yemen and disease epidemiology among orphaned children is not well known. Consequently, the current study was aimed to determine the prevalence of Hepatitis B infection among children living in the orphanage in Sana’a city, Yemen. A cross-sectional descriptive study was conducted from March to May 2021 to determine the HBsAg seroprevalence among children living in orphanage in Sana’a city. Three hundred and thirty-three (333) blood specimens were randomly collected from orphan children and the HBsAg was determined by using an enzyme-linked immunosorbent assay (ELISA). Also, the needed data and associated risk factors were collected by a standard questionnaire. The results revealed that the rate of HBsAg seroprevalence was (0.0%) recorded among participated children which means all of them are free of HBV infection. The rest of the factors failed to achieve any statistical significance with the seroprevalence of HBsAg. Moreover, the most who participated in this study were children in the age group 11-15 years old, not having work and family history of HBV, didn’t smoke, medication, shared a toothbrush with another person, and infected relative HBV. Also, the vast majority of all participants had no risk factors parameters for HBV according to hemodialysis, blood transfusion, organ transplantation, cupping, and vaccination. It is recommended that the implementation of an effective health program such as hepatitis B vaccination and health education would further reduce the prevalence of hepatitis B.



Hepatitis B virus,




Sana’a, Yemen.

Authors’ Contribution

AMB and GHA conceived and designed the study; AMB and WAA did literature search; GHA performed clinical trials; AMB and GHA performed experiments; WE and WAA did data analysis; AMB and WE wrote and revised the paper; AMB and WAA review the manuscript.

How to cite

Banafa, A.M., Edrees, W.H., Al-Falahi, G.H., Al-Shehari, W.A., 2022. Prevalence of Hepatitis B Surface Antigen among Orphans Children Living in Orphanage in Sana’a City, Yemen. PSM Microbiol., 7(1): 19-26.


Abdullah, Q.Y., Al-Helali, M.F., Al-Mahbashi, A., Qaaed, S.T., Edrees, W.H., 2020. Seroprevalence of dengue fever virus among suspected patients in Taiz Governorate-Yemen. Univers. J. Pharm. Res., 5(5):21-26.

Al-Haik, M.W., Al-Haddad, M.A., Al-Kaf, G.A., Edrees, H.W., 2017. Antimicrobial activities for hadhrami honey on growth of some pathogenic bacteria. Univers. J. Pharm. Res., 2(6): 7-12.

Alhlale, F.M., Saleh, H.A., Alsweedi, S.K., Edrees, H.W., 2019. The inhibitory effect of Euphorbia hirta extracts against some wound bacteria isolated from Yemeni patients. COPS. 3(2): 780-786.

Alhlale, F.M., Humaid, A., Saleh, A.H., et al., 2020. Effect of most common antibiotics against bacteria isolated from surgical wounds in Aden Governorate hospitals, Yemen. Univers. J. Pharm. Res., 5(1): 21-24.

Al-Khawlany, R.S., Edrees, W.H., AL-Jaufy, A.Y., et al., 2021. Prevalence of methicillin-resistant Staphylococcus aureus and antibacterial susceptibility among patients with skin and soft tissue infection at Ibb city, Yemen. PSM Microbiol., 6(1): 1-11.

Almezgagi, M.M., Edrees, W.H., Al-Shehari, W.A., Al-Moyed, K., Al-Khwlany, R.S., Abbas, A.B., 2020. Prevalence of hepatitis B virus and hepatitis C virus and associated risk factors among hemodialysis patients in Ibb city-Yemen. PSM Microbiol., 5(2): 32-40.

Al-Ofairi, B.A, Humaid, A.A, Al-Huthaifi, A.M. Serum HBe Ag and HBV DNA markers among different stages of chronic hepatitis B patients in Sana’a city, Yemen. I.J.I.S.R.T., 5(3): 625-634.

Alshahethi, M.A., Edrees, W.H., Mogalli, N.M., Al-Halani, A.A., 2020. Prevalence of Entamoeba histolytica among children attending healthcare centres at Amran governorate, Yemen. PSM Biol. Res., 5(3): 98-105.

Al-Shamahy, H., 2000. Prevalence of hepatitis B surface antigen and risk factors of HBV infection in a sample of healthy mothers and their infants in Sana’a, Yemen. Ann. Saudi Med., 20(5-6):464-466.

Al-Shamahy, H.A., Hanash, S.H., Rabbad, I.A.,  Al-Madhaji, N.M., Naser, SM., 2011. Hepatitis B vaccine coverage and the immune response in children under ten years old in Sana’a, Yemen. SQU. Med. J., 11(1): 77-82.

Al-Shamahy, H.A., Jaadan, B.M., Al-Madhaji, A.G., et al., 2019. Prevalence and potential risk factors of hepatitis B virus in a sample of children in two selected areas in Yemen. Univers. J. Pharm. Res., 4(3): 17-21.

Al-Shamahy, H.A., Rabbad, I.A., Al-Hababy, A., 2003. Hepatitis B virus serum markers among pregnant women in Sana’a, Yemen. Ann. Saudi. Med., 23:87−89.

Bakthavatchalu, S., 2012. Hepatitis B surface antigen carrier state among asymptomatic pregnant women and its correlation with vertical transmission. Int. J. Res. Pharm. Sci., 2(3):111–120.

Bawazir,A.A., Christopher, M., Parry, C.M., et al., 2010. Seroepidemiology and risk factors of hepatitis B virus in Aden, Yemen. J. Infect. Public. Health., 4(1):48-54.

Camvulam, N., Gotsch, P., Langan, R.C., 2010. Caring for pregnant women and newborns with hepatitis B or C. Am. Fam. Physician., 82(10):1225–1229.

Dilshad, F., Irfan, M., Qayyum, M., Shabbir, A., Ashraf, A., Iqbal, A., Iqbal, M.N., Muhammad, A., 2016. Incidence of Hepatitis B among Malarial Patients in Islamabad. PSM Biol. Res., 1(S1): S6-S8.

Edrees, H.W., Al-Awar S.M., 2020. Bacterial contamination of mobile phones of medical laboratory workers at Sana’a city, Yemen and their antimicrobial susceptibility. J.P.P.Res., 8 (6): 591-599.

Edrees, H.W., Anbar, A.A., 2020. Prevalence and antibacterial susceptibility of bacterial uropathogens isolated from pregnant women in Sana’a, Yemen. PSM Biol Res., 5(4): 157-165.

Edrees, W,H., 2021. Antibacterial susceptibility and Sider honey activity against isolated bacteria from wound patients attending at Al-Gmohori hospital in Hajja city, Yemen. Al-Razi. Univ. J. Med. Sci., 5 (2):1-8.

Edrees, W.H., Al-Ofairi, B.A., Alsaifi, A.G., et al., 2022. Prevalence of intestinal parasitic infections among asymptomatic primary schoolchildren at Al-Sabeen district in Sana’a city, Yemen. PSM Biol. Res., 7(1): 34-45.

Edrees, W.H., Anbar, A.M., 2021. Prevalence and antibiotic susceptibility of Streptococcus pyogenes isolated from schoolchildren in Sana’a city, Yemen. PSM Vet. Res., 6(2):  22-30.

Edrees, W.H., Mogalli, N.M., Alabdaly, K.W., 2021. Assessment of some clinical and laboratory profiles among dengue fever patients in Hajjah government, Yemen. UJPR., 6(2):38-41.

Gacche, R.N., Kaid, A.S., 2012. Epidemiology of viral hepatitis B and C infections in Ibb city, Yemen. Hepat. Mon., 12(7): 460–462.

Gobara’a, A.A., Edrees, W.H., Al-Shehari, W.A., et al., 2020. Prevalence of rubella IGg antibodies among productive-age women in Al-Mahweet governorate, Yemen. U.J.P.R., 5(4):28-32.

Haukenes, G., Brinchmann-Hansen, K., Macovei, O., 1992. Prevalence of hepatitis B and C and HIV antibodies in children in a Romanian orphanage. A.P.M.I.S., 100: 757-761.

Hussien, M., Zahran, F., El-Emshaty, H., Gouida, M.S., Iqbal, M.N., 2018. Diagnostic Value of Fibrotic Indices in Egyptian Patients with Chronic Hepatitis and Hepatocellular Carcinoma. PSM Biol. Res., 3(1): 9-15.

International Agency for Research on Cancer (IARC), 2021. Agents classified by the IARC monographs. 2012. Available:    Accessed 31 June 2022.

Iqbal, M.N, Ashraf, A., Iqbal, I., 2019. Hepatitis C Virus Infection: Evaluation of ICT and ELISA for the Screening of anti-HCV. Int. J. Mol. Microbiol., 2(2): 29-40.

Kalim, M., Ahmad, H., Rauf, A., Nadeem, S., Rahat, M.A., Din, S.U., 2017a. Incidence of HBV and HCV in Young Orphan Students of Swat. PSM Biol. Res., 2(3): 128-132.

Kalim, M., Imran, M., Hussain, F., Khan, IU., Habib, N., Iqbal, M.N, Ashraf, A., 2017b. Detection of HBV and HCV by ICT and ELISA Method in Different Areas of District Malakand. PSM Microbiol., 2(1): 5-8.

Mogalli, N.M., Edrees,W.H., Al-Awar, M.S., Alshahethi, M.A., Al-Shehari, W.A., 2020. Prevalence of intestinal parasitic infections among primary schoolchildren in Kohlan district at Hajjah governorate, Yemen. Al-Razi. Univ. J. Med. Sci., 4 (2):34-39.

Ogbonna, C.P., Ogoke, C.C., Ikefuna, A.N., Ezeofor, T.C., Nwolisa, E.C., et al., 2021. Risk factors, seroprevalence and infectivity of hepatitis B virus amongst children resident in orphanages in a developing country. J. Infect. Dis. Epidemiol., 7:186.

Oluboyo, B.O., Enweani, I.B., Ekejindu, I.M., Oluboyo, A.O., 2014. HIV and hepatitis infections in children resident in orphanage homes located in Anambra State, Nigeria. IOSR J. Nurs. Heal. Sci., 3: 47-50.

Ork, V., Woodring, J., Hossain, Md, S., et al., 2019. Hepatitis B surface antigen seroprevalence among pre- and post-vaccine cohorts in Cambodia, 2017. Vaccine., 14; 37(35): 5059–5066.

Paquet, C., Babes, V.T., Drucker, J., Senemaud, B., Dobrescu, A., 1993. Viral hepatitis in Bucharest. Bull. World. Health. Organ., 71(6):781-786.

Sallam, T.A., Tong, C.Y., Cuevas, L.E., Raja’a, Y.A., Othman, A.M., Al-Kharsa, K.R., 2003. Prevalence of blood-borne viral hepatitis in different communities in Yemen. Epidemiol. Infect., 131:771-775.

Toor, S., Toor, S., Ashraf, A., Alam, S., Anwaar, S., Saddiqa, A., Ali, S., Muhammad, A., Toor, S., Akhter, S., 2016. Prevalence of Liver disorders in Islamabad City. PSM Biol. Res., 01(1): 31-33.

World Health Organization (WHO) 2016a. Global health estimates. Deaths by cause, age, sex, by country and by region, 2000–2015. Geneva: World Health Organization. 2015.

World Health Organization (WHO) 2016b. World health statistics 2016: monitoring health for the SDGs sustainable development goals: World Health Organization; 2016.

World Health Organization (WHO) 2021. Hepatitis B. 27 July 2021. avialable at