Prevalence of Hepatitis B Surface Antigen among Orphans Children Living in Orphanage in Sana’a City, Yemen

Author's: Amal Mohammed Banafa1, Wadhah Hassan Edrees1,2*, Ghada Hassein Al-Falahi3, Wadee Abdullah Al-Shehari4
Authors' Affiliations
1Medical Laboratory Department, Faculty of Medical Sciences, Al-Razi University, Yemen.2Medical Microbiology Department, Faculty of Applied Sciences, Hajjah University, Yemen.3Medical Laboratory Department, Faculty of Medical Sciences, Sana'a University, Yemen.4Department of Microbiology, Faculty of Medical Sciences, Ibb University, Yemen.
*CorrespondenceWadhah Hassan;
Article Type: Research Article     Published: Mar. 17, 2022 Pages: 19-26
DOI:        Views 512       Downloads 0


Hepatitis B virus (HBV) is considered a serious public health issue in Yemen and disease epidemiology among orphaned children is not well known. Consequently, the current study was aimed to determine the prevalence of Hepatitis B infection among children living in the orphanage in Sana’a city, Yemen. A cross-sectional descriptive study was conducted from March to May 2021 to determine the HBsAg seroprevalence among children living in orphanage in Sana’a city. Three hundred and thirty-three (333) blood specimens were randomly collected from orphan children and the HBsAg was determined by using an enzyme-linked immunosorbent assay (ELISA). Also, the needed data and associated risk factors were collected by a standard questionnaire. The results revealed that the rate of HBsAg seroprevalence was (0.0%) recorded among participated children which means all of them are free of HBV infection. The rest of the factors failed to achieve any statistical significance with the seroprevalence of HBsAg. Moreover, the most who participated in this study were children in the age group 11-15 years old, not having work and family history of HBV, didn’t smoke, medication, shared a toothbrush with another person, and infected relative HBV. Also, the vast majority of all participants had no risk factors parameters for HBV according to hemodialysis, blood transfusion, organ transplantation, cupping, and vaccination. It is recommended that the implementation of an effective health program such as hepatitis B vaccination and health education would further reduce the prevalence of hepatitis B.



Hepatitis B virus,




Sana’a, Yemen.

Authors’ Contribution

AMB and GHA conceived and designed the study; AMB and WAA did literature search; GHA performed clinical trials; AMB and GHA performed experiments; WE and WAA did data analysis; AMB and WE wrote and revised the paper; AMB and WAA review the manuscript.

How to cite

Banafa, A.M., Edrees, W.H., Al-Falahi, G.H., Al-Shehari, W.A., 2022. Prevalence of Hepatitis B Surface Antigen among Orphans Children Living in Orphanage in Sana’a City, Yemen. PSM Microbiol., 7(1): 19-26.


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