Phytochemical Screening, Antimicrobial, Antioxidant and Anticancer Activities of Yemeni Croton socatranus

Author's: Ibrahim Ali Al-Hakami1, Amina El-Shaibany1, Hassan Al-Mahbashi2 , Abdulrahman Humaid3,4*, Salwa Raweh1,   Mahmoud M. Elaasser5
Authors' Affiliations
1Pharmacognosy Department, Pharmacy College, University of Sana’a, Yemen.2Department of Forensic Medicine and Clinical Toxicology, College of Medicine, Sana`a University, Sanaa, Yemen.3Department of Biology, Faculty of Science, Sana`a University, Sanaa, Yemen.4Department of Pharmacy, Faculty of Medical Science, Modern Science University, Sana`a, Yemen.5The Regional Center for Mycology and Biotechnology, Al-Azhar University, 11787 Nasr City, Cairo, Egypt.
*CorrespondenceAbdulrahman Humaid
Article Type: Research Article     Published: Feb. 28, 2022 Pages: 1-13
DOI:        Views 200       Downloads 0


Croton socatranus is a shrub or small tree endemic to Socotra Island and is widespread on the island. It has some interesting ethnomedicinal uses. The dry powder was subjected to extraction till exhaustion using methanol. The methanol extract was evaluated phytochemically, chromatographically, and for its antimicrobial and antioxidant activity. Toxicological and cytotoxic effects were also investigated. Microscopic examination of C. socatranus aerial parts confirmed the presence of xylem vessels, sclerenchymatous cells, slender-shaped fibers, vessel elements with scalariform thickenings, palisade cells, paracytic stomata, and stellate trichomes. Phytochemical investigation showed that the methanol extract possessed alkaloids, high levels of phenolic compounds, tannins, flavonoids, triterpenes, diterpenes, phytosterol, and saponin. Notably, the methanol extract of C. socatranus did not cause any mortality or behavioral changes in the rats even at the highest tested dose (5000mg/kg of methanol extract). It showed inhibition zone against Streptococcus pneumonia and Escherichia coli and MIC values were between 156.25- 655 µg/ml for gram-positive strains while 625-2500 µg/ml for gram-negative bacteria. Antifungal activity was recorded against ten tested fungal species. Furthermore, a dose-dependent antioxidant activity was observed. C. socatranus extract displayed moderate cytotoxic activity against eight cancerous cell lines. The extract was highly effective on MCF-7, HepG-2, and RD cell lines when compared with other cell lines and the IC50 was less than 30 µg/ml which suggests the presence of selective cytotoxic compounds. Further investigations are required to understand the possible mechanism(s) of action of this extract on various cancer cells and the isolation of active phytochemicals.


Croton socatranus,

Acute oral toxicity,




Authors’ Contribution

AE conceived and designed the experiment, AE, SR, IAA, HA, AH and MEE carried out the experiments, and MEE analyzed data, AE and IAA wrote the article, AE gave final approval.

How to cite

Al-Hakami, I.A., El-Shaibany, A., Al-Mahbashi, H., Humaid, A., Raweh, S., Elaasser, M.M., 2022. Phytochemical Screening, Antimicrobial, Antioxidant and Anticancer Activities of Yemeni Croton socatranus. Int. J. Nanotechnol. Allied Sci., 6(1): 1-13.


Abdelrahman, M.A., Salama, I., Gomaa, M.S., Elaasser, M.M., Abdel-Aziz, M.M., Soliman, D.H., 2017. Design, synthesis and 2D QSAR study of novel pyridine and quinolone hydrazone derivatives as potential antimicrobial and antitubercular agents. Eur. J. Med. Chem., 138: 698-714.

Bantie, L., Assefa, S., Teklehaimanot, T., Engidawork, E., 2014. In vivo antimalarial activity of the crude leaf extract and solvent fractions of Croton macrostachyus Hocsht. (Euphorbiaceae) against Plasmodium berghei in mice. BMC Complement. Altern. Med., 14(1): 1-10.

Banu, K.S., Cathrine, L., 2015. General techniques involved in phytochemical analysis. Int. J. Adv. Res. Chem. Sci., 2(4): 25-32.

Caruzo, M.B., 2013. Taxonomic revision of Croton section Cleodora (Euphorbiaceae). Phytotaxa, 121: 1-41.

de Sá-Haiad, B., Serpa-Ribeiro, A., Barbosa, C., Pizzini, D., de O Leal, D., de Senna-Valle, L., de Santiago-Fernandes, L., 2009. Leaf structure of species from three closely related genera from tribe Crotoneae Dumort.(Euphorbiaceae ss, Malpighiales). Plant System. Evol., 283(3): 179-202.

De Sanctis, M., Adeeb, A., Farcomeni, A., Patriarca, C., Saed, A., Attorre, F., 2013. Classification and distribution patterns of plant communities on Socotra Island, Yemen. App. Veget. Sci., 16(1): 148-165.

Dhole, J., Dhole, N., Lone, K., Bodke, S., 2012. Preliminary phytochemical analysis of weeds in Marathwada region. Res. J. Pharmac. Biol. Chem. Sci., 3(4): 764-767.

Divya, S., Krishna, K.N., Ramachandran, S., Nagar, C., 2011. Wound Healing and In Vitro Antioxidant Activities of Croton bonplandianum Leaf Extract in Rats.

Dutta, S., Dey, P., Chaudhuri, T.K., 2014. Phytochemical investigation and correlation study of Croton bonplandianus Baill. stem. J. Pharmac. Phytochem., 3(1).

El Gamal, A.A., 2010. Biological importance of marine algae. Saudi Pharmac. J., 18(1): 1-25.

Hodge, A., Sterner, B., 2005. Toxicity classes in: Canadian Centre for occupational health and Safety. Copyright@ 1997–2010.

Hussain, F., Kalim, M., Ali, H., Ali, T., Khan, M., Xiao, S., Iqbal, M., Ashraf, A., 2016. Antibacterial activities of methanolic extracts of Datura inoxia. PSM Microbiol., 1(1): 33-35.

Iqbal, M.N., Anjum, A.A., Ali, M.A., Hussain, F., Ali, S., Muhammad, A., Irfan, M., Ahmad, A., Irfan, M., Shabbir, A., 2015. Assessment of Microbial Load of Un-pasteurized Fruit Juices and in vitro Antibacterial Potential of Honey Against Bacterial Isolates. Open Microbiol. J., 9: 26-32.

Kalim, M., Hussain, F., Ali, H., Iqbal, M., 2016. Antifungal activities of Methanolic Extracts of Datura inoxia. PSM Biol. Res., 1(2): 70-73.

Klimczak, I., Małecka, M., Szlachta, M., Gliszczyńska-Świgło, A., 2007. Effect of storage on the content of polyphenols, vitamin C and the antioxidant activity of orange juices. J. Food Compost. Anal., 20(3-4): 313-322.

Kumar, A., Jha, A., 2017. Chapter 7 – Drug Development Strategies. In: Kumar, A., Jha, A. (Eds.), Anticandidal Agents. Academic Press, pp. 63-71.

Lee, S.E., Hwang, H.J., Ha, J.-S., Jeong, H.-S., Kim, J.H., 2003. Screening of medicinal plant extracts for antioxidant activity. Life Sci., 73(2): 167-179.

Mbiantcha, M., Nguelefack, T., Ndontsa, B., Tane, P., Kamanyi, A., 2013. Preliminary assessment of toxicity of Croton macrostachyus stem bark (Euphorbiaceae) extracts. Int. J. Pharm. Chem. Biol. Sci., 3: 2-113.

Miller, A.G., Morris, M., 2004. Ethnoflora of the Soqotra Archipelago. Royal Botanic Garden Edinburgh.

Mulcahy, N., 2008. Cancer to become leading cause of death worldwide by 2010. Medscape Medical News.

Nath, R., Roy, S., De, B., Choudhury, M.D., 2013. Anticancer and antioxidant activity of croton: a review. Int. J. Pharm. Pharm. Sci., 5(2): 63-70.

National Institutes of Health, N., 1986. Office for Protection from Research Risks. Public Health Service policy on humane care and use of laboratory animals. Office for Protection from Research Risks (OPRR), National Institutes of Health.

Newman, D.J., Cragg, G.M., 2014. Marine-sourced anti-cancer and cancer pain control agents in clinical and late preclinical development. Mar. Drugs, 12(1): 255-278.

Nigussie, D., Davey, G., Legesse, B.A., Fekadu, A., Makonnen, E., 2021. Antibacterial activity of methanol extracts of the leaves of three medicinal plants against selected bacteria isolated from wounds of lymphoedema patients. BMC Complem. Med. Therap., 21(1): 2.

Onwusonye, J., Muritala, I., Odu, D., Uzomba, N., Ogbonnaya, M., Duruamaku, C., 2016. Oral acute toxicity study of methanol leaf extracts of Croton zambesicus in mice. Merit. Res. J. Pharm. Pharm. Sci., 2: 1-5.

Sabir, S.M., Hayat, I., Gardezi, S.D.A., 2003. Estimation of sterols in edible fats and oils. Pak. J. Nutr., 2(3): 178-181.

Selowa, S.C., Shai, L.J., Masoko, P., Mokgotho, M.P., Magano, S., 2010. Antibacterial activity of extracts of three Croton species collected in Mpumalanga region in South Africa. Afr. J. Trad. Complem. Altern. Med., 7(2).

Seoudi, D., Medhat, A., Hewedi, I., Osman, S., Mohamed, M.K., Arbid, M., 2009. Evaluation of the anti-inflammatory, analgesic, and anti-pyretic effects of Origanum majorana ethanolic extract in experbimental animals. J. Radiation Res. App. Sci., 2(3): 513-534.

Smibert, R., Krieg, N., Gerhardt, P., Murray, R., Wood, W., 1994. Methods for general and molecular bacteriology. American Society for Microbiology, Washington, DC: 607-654.

Subhasree, B., Baskar, R., Keerthana, R.L., Susan, R.L., Rajasekaran, P., 2009. Evaluation of antioxidant potential in selected green leafy vegetables. Food Chem., 115(4): 1213-1220.

Suffness, M., 1990. Assays related to cancer drug discovery. Methods in plant biochemistry: assays for bioactivity, 6: 71-133.

Suurbaar, J., Mosobil, R., Donkor, A.-M., 2017. Antibacterial and antifungal activities and phytochemical profile of leaf extract from different extractants of Ricinus communis against selected pathogens. BMC Res. Notes., 10(1): 660.

Tardelli, M., Baldini, R., 2000. Botanical report on the island of Socotra (Yemen). Portugaliae Acta Biologica, ISSN 0874-9035, Vol. 19, nº 1-4, 2000, pags. 443-454.

Van Acker, S.A., Tromp, M.N., Griffioen, D.H., Van Bennekom, W.P., Van Der Vijgh, W.J., Bast, A., 1996. Structural aspects of antioxidant activity of flavonoids. Free Rad. Biol. Med., 20(3): 331-342.

Wagate, C.G., Mbaria, J.M., Gakuya, D.W., Nanyingi, M.O., Kareru, P., Njuguna, A., Gitahi, N., Macharia, J.K., Njonge, F.K., 2010. Screening of some Kenyan medicinal plants for antibacterial activity. Phytother. Res., 24(1): 150-153.

Yang, H., Zeng, M., Dong, S., Liu, Z., Li, R., 2010. Anti-proliferative activity of phlorotannin extracts from brown algae Laminaria japonica Aresch. Chin. J. Oceanol. Limnol., 28(1): 122-130.

Yen, G.C., Duh, P.D., 1994. Scavenging effect of methanolic extracts of peanut hulls on free-radical and active-oxygen species. J. Agric. Food Chem., 42(3): 629-632.

Zahid, K., Mughal, T., 2019. Evaluation of Antifungal Activity of Nine Members of Family Euphorbiaceae of Lahore Region against Aspergillus niger, Rhizopus oryzae & Alternaria solani. Am. J. Biomed. Sci. Res., 6(3): 205-208.

Zhishen, J., Mengcheng, T., Jianming, W., 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem., 64(4): 555-559.