PCR and Nanotechnology Unraveling Detection Problems of the Seed-borne Pathogen Cephalosporium maydis, the Causal Agent of Late Wilt Disease in Maize

Author's: Awad, M.A., I.H. El-Abbasi, Tahsin Shoala, Sahar, A. Youssef, Dalia M. Shaheen, Gomaa, A. Amer

Corresponding Author: Tahsin Shoala

Corresponding Author Email: tahsinshoala2000@gmail.com

Article Type: Research Article

Published Online: May. 31, 2019

Pages: Online first

Views: 56

Downloads: 0

Abstract:

The current study was conducted to access the role of PCR and Nanotechnology unraveling detection problems of the seed-borne pathogen Cephalosporium maydis. A total of four maize (Zea maize L.) seed samples were collected from fields known with a long history of heavy infection of late wilt disease caused by the fungus Cephalosporium mayids (Harpophora maydis). The detection and isolation of C. maydis associated with maize seeds were carried out using the blotter, deep freezing and agar plate methods followed by the application of metallic nanoparticles to enhance the DNA extraction methods compared to standard protocols.

Read more

Keywords:

Maize, seed-borne, late wilt, Cephalosporium maydis, nano-particles, PCR.

Citation:

Awad, M.A., El-Abbasi, I.H., Shoala, T., Youssef, S.A., Shaheen, D.M., Amer, G.A., 2019. PCR and Nanotechnology Unraveling Detection Problems of the Seed-borne Pathogen Cephalosporium maydis, the Causal Agent of Late Wilt Disease in Maize. Int. J. Nanotechnol. Allied Sci., 3(2): IJNAS-2019-021.

PDF: