Fluorescence Activated Cell Sorting (FACS): An Advanced Cell Sorting Technique
Views 2760
Abstract:
Fluorescence-activated cell sorting (FACS) follows the principle of flow cytometry. It is used to separate the molecules or cells of interest from the heterogeneous mixture by selecting the specific cells. The sample is prepared by labelling the cells with fluorescence dye. It is excited by a laser source which results in scattering and fluorescence due to absorbed light. A number of systems have been produced using two or three lasers to excite several dyes. The light scatter and fluorescence detector outputs are amplified and processed for evaluation. The function of fluorescent excitation and detection system is to produce the signals which accurately reflect the amount of each dye associated with the cell. It is applied in the biological research field, i.e. lymphocyte determination, isolation and characterization of infected stem cells, screening of protein binding with various ligands and for the quantification of nucleic acid content in a cell.
Keywords: Fluorescence-activated cell sorting (FACS); signal detection and amplification; proteins; stem cells; nucleic acid.
Cite this article: Naeem, A., James, N., Tanvir, M., Marriam, M., Nathaniel, S., 2017. Fluorescence Activated Cell Sorting (FACS): An Advanced Cell Sorting Technique. PSM Biol. Res., 2(2): 83-88.
Related Content
Quick Links
Announcements
Call for papers: The PSM – Multidisciplinary Scientific Journal
Call for papers: PSM Veterinary Research
Call for papers: International Journal of Alternative Fuels and Energy
Call for papers: International Journal of Nanotechnology and Allied Sciences
Call for papers: PSM Biological Research
Call for papers: International Journal of Molecular Microbiology
Call for papers: PSM Microbiology
Letter from the Editor of Publications
Antibacterial Compounds from Ethanolic Extract of Scenedesmus obliquus as Alternatives to Antibiotics
Bioactivity of Natural Compounds Extracted from Scenedesmus obliquus toward Some Pathogenic Bacteria
Early Feed Restriction Can Affect the Behavior and Welfare of Mule Ducks
The Impact of Stress on Laboratory Animals and Variability in Research Outcome