Fabrication and Characterization of Antibacterial Biodegradable Polymeric Nanofibers of Polyvinyl Alcohol loaded with Levofloxacin for External Skin Infection

Author's: Ghulam Murtaza Khuhro*, M. Aqeel Bhutto, Adeela Chachar, Mahesh Kumar
Authors' Affiliations
Institute of Biotechnology and Genetic Engineering, University of Sindh Jamshoro, Pakistan.
*CorrespondenceGhulam Murtaza Khuhro Email:gmurtaza.khuhro@usindh.edu.pk
Article Type: Research Article     Published: Mar. 28, 2022 Pages: 14-22
DOI:        Views 190       Downloads 0


Electrospun nanofibrous membranes have gained great focus in medical research due to its biocompatibility and biodegradability. Research proposed that it is most advanced method of delivering drug to patient’s body at a particular site of infection. Through this targeted delivery, there is increased rate of delivered drug to an infected body part; it may be organs, tissues or cells. As result, the action of recovery of infection is enhanced without causing any side effects. During present study sustainable electrospun nanofibers were fabricated via biodegradable synthetic polymer polyvinyl alcohol (PVA) loaded with antibiotic (Levofloxacin). Control (PVA) and antibiotic loaded (PVA/LVF) nanofibers were synthesized through simple electrospinning. Synthesized materials were characterized through scanned electron microscopy (SEM) for morphology that showed average fiber diameter size of 146.24±44.024 µm and 184.79±41.94 µm respectively. While for chemical characterization FTIR was carried out. Further antibacterial susceptibility was checked against Escherichia coli for different time period of incubation and maximum zone of inhibition was observed at 72 hours that was 29±0.25 mm as compared to 24 h and 48 h. While release rate of drug in artificial medium phosphate buffer saline (PBS) was measured by spectrophotometric method up to 72 hours. In the medium at initial stage nano fibers showed burst release of drug up to 24 hour, later on sustain release behavior was observed up to 72 h. From the findings it was suggested that synthesized material especially used in the synthesis of gauze for external skin infections, it could also be used in the synthesis of sutures, wound patches bandages and other biomedical applications.



Poly Vinyl Alcohol,


Bacterial susceptibility,

Sustained drug release.

Authors’ Contribution

GMK conceived and designed the experiment, GMK, AC and MK carried out the experiments; GMK analyzed data and wrote the article; MAB gave final approval.

How to cite

Khuhro, G.M., Bhutto, M.A., Chachar, A., Kumar, M., 2022. Fabrication and Characterization of Antibacterial Biodegradable Polymeric Nanofibers of Polyvinyl Alcohol loaded with Levofloxacin for External Skin Infection. Int. J. Nanotechnol. Allied Sci., 6(1): 14-22.


Babu, M., 2000. Collagen based dressings—a review. Burns, 26(1): 54-62.

Bhutto, M.A., Wu, T., Sun, B., Hany, E.-H., Al-Deyab, S.S., Mo, X., 2016a. Fabrication and characterization of vitamin B5 loaded poly (l-lactide-co-caprolactone)/silk fiber aligned electrospun nanofibers for schwann cell proliferation. Colloids and Surfaces B: Biointerfaces, 144: 108-117.

Bhutto, M.A., Zhang, J., Sun, B., El-Hamshary, H., Al-Deyab, S.S., Mo, X., 2016b. Development of poly (L-lactide-co-caprolactone) multichannel nerve conduit with aligned electrospun nanofibers for Schwann cell proliferation. International Journal of Polymeric Materials and Polymeric Biomaterials, 65(7): 323-329.

Boateng, J.S., Matthews, K.H., Stevens, H.N., Eccleston, G.M., 2008. Wound healing dressings and drug delivery systems: a review. Journal of pharmaceutical sciences, 97(8): 2892-2923.

El-Shanshory, A.A., Chen, W., El-Hamshary, H.A., Al-Deyab, S.S., Mo, X., 2015. Antibacterial ciprofloxacin hydrochloride incorporated PVA/regenerated silk fibroin nanofibers composite for wound dressing applications. Journal of controlled release: official journal of the Controlled Release Society, 213: e8.

Hill, K.J., Kaszuba, M., Creeth, J.E., Jones, M.N., 1997. Reactive liposomes encapsulating a glucose oxidase-peroxidase system with antibacterial activity. Biochimica et Biophysica Acta (BBA)-Biomembranes, 1326(1): 37-46.

Jalvandi, J., White, M., Gao, Y., Truong, Y.B., Padhye, R., Kyratzis, I.L., 2017. Polyvinyl alcohol composite nanofibres containing conjugated levofloxacin-chitosan for controlled drug release. Materials Science and Engineering: C, 73: 440-446.

Kalwar, K., Bhutto, M.A., Dali, L., Shan, D., 2017. Cellulose based nanofabrication; immobilization of silver nanoparticales and its size effect against Escherichia coli. Materials Research Express, 4(10): 105405.

Ketabchi, N., Naghibzadeh, M., Adabi, M., Esnaashari, S.S., Faridi-Majidi, R., 2017. Preparation and optimization of chitosan/polyethylene oxide nanofiber diameter using artificial neural networks. Neural Computing and Applications, 28(11): 3131-3143.

Leeds, I.L., Fabrizio, A., Cosgrove, S.E., Wick, E.C., 2017. Treating wisely: the surgeon’s role in antibiotic stewardship. Annals of surgery, 265(5): 871.

Liu, H., Mulholland, S.G., 2005. Appropriate antibiotic treatment of genitourinary infections in hospitalized patients. The American journal of medicine, 118(7): 14-20.

Ma, Z., Kotaki, M., Inai, R., Ramakrishna, S., 2005. Potential of nanofiber matrix as tissue-engineering scaffolds. Tissue engineering, 11(1-2): 101-109.

MacDougall, C., Guglielmo, B.J., Maselli, J., Gonzales, R., 2005. Antimicrobial drug prescribing for pneumonia in ambulatory care. Emerging infectious diseases, 11(3): 380.

Nelson, J.M., Chiller, T.M., Powers, J.H., Angulo, F.J., 2007. Fluoroquinolone-resistant Campylobacter species and the withdrawal of fluoroquinolones from use in poultry: a public health success story. Clinical Infectious Diseases, 44(7): 977-980.

Pelipenko, J., Kocbek, P., Kristl, J., 2015. Critical attributes of nanofibers: Preparation, drug loading, and tissue regeneration. International journal of pharmaceutics, 484(1-2): 57-74.

Pham, Q.P., Sharma, U., Mikos, A.G., 2006. Electrospinning of polymeric nanofibers for tissue engineering applications: a review. Tissue engineering, 12(5): 1197-1211.

Pouton, C.W., Porter, C.J., 2008. Formulation of lipid-based delivery systems for oral administration: materials, methods and strategies. Advanced drug delivery reviews, 60(6): 625-637.

Rani, K., Paliwal, S., 2014. A review on targeted drug delivery: Its entire focus on advanced therapeutics and diagnostics. Sch. J. App. Med. Sci, 2(1C): 328-331.

Schaeffer, A., 2003. Levofloxacin: an updated review of its use in the treatment of bacterial infections. The Journal of urology, 170(1): 337.

Schiffman, J.D., Schauer, C.L., 2008. A review: electrospinning of biopolymer nanofibers and their applications. Polymer reviews, 48(2): 317-352.

Shin, H., 2007. Fabrication methods of an engineered microenvironment for analysis of cell–biomaterial interactions. Biomaterials, 28(2): 126-133.

Sill, T.J., Von Recum, H.A., 2008. Electrospinning: applications in drug delivery and tissue engineering. Biomaterials, 29(13): 1989-2006.

Sudhakar, C., Upadhyay, N., Verma, A., Jain, A., Charyulu, R.N., Jain, S., 2015. Nanomedicine and tissue engineering, Nanotechnology Applications for Tissue Engineering. Elsevier, pp. 1-19.

Sun, B., Li, J., Liu, W., Aqeel, B.M., El-Hamshary, H., Al-Deyab, S.S., Mo, X., 2015. Fabrication and characterization of mineralized P (LLA-CL)/SF three-dimensional nanoyarn scaffolds. Iranian Polymer Journal, 24(1): 29-40.

Trivedi, P., Vasudevan, D., 2007. Spectroscopic investigation of ciprofloxacin speciation at the goethite− water interface. Environmental science & technology, 41(9): 3153-3158.

Vrbata, P., Berka, P., Stránská, D., Doležal, P., Musilová, M., Čižinská, L., 2013. Electrospun drug loaded membranes for sublingual administration of sumatriptan and naproxen. International journal of pharmaceutics, 457(1): 168-176.

Xie, J., Li, X., Xia, Y., 2008. Putting electrospun nanofibers to work for biomedical research. Macromolecular rapid communications, 29(22): 1775-1792.

Yang, F., Murugan, R., Wang, S., Ramakrishna, S., 2005. Electrospinning of nano/micro scale poly (L-lactic acid) aligned fibers and their potential in neural tissue engineering. Biomaterials, 26(15): 2603-2610.

Zhao, Y., Yang, Q., Lu, X.-F., Wang, C., Wei, Y., 2005. Study on correlation of morphology of electrospun products of polyacrylamide with ultrahigh molecular weight. Journal of Polymer Science Part B: Polymer Physics, 43(16): 2190-2195.