Elucidative Physiological Optimization of Silver Nanospheres Biogenesis by Molds

Author's: Amal, A.I. Mekawey and Helmy E. A.
Authors' Affiliations
Article Type: Research Article     Published: Nov. 23, 2017 Pages: 30-44
DOI:        Views 1554       Downloads 0


One of the substantial and most ordinary requests asked for that when starting to oversee nanoparticles is “The reason is nanoparticles so intriguing? Why work with these to an incredible degree little structures that are attempting to manage and join especially when differentiated and their obviously noticeable accomplices? The suitable reaction lies in the novel properties controlled by these nanoparticles. In vitro myco synthesis of silver nanoparticles (AgNPs) using Penicillium aurantiogresium, Penicillium roqueforti, Aspergillus niger, Verticillium chlamydosporium var. chlamydosporium, Trichoderma viride and Trichoderma longibranchiatum had been investigated. The procedure of silver particle lessening by either extracellular contagious filtrate or intracellular without cell filtrate was accomplished which prompt the improvement of an easy procedure for the amalgamation of silver nanoparticles. Upon exposure of the fungal filtrate to silver nitrate, the latter was reduced to silver nanoparticles as indicated by a color change observed and characterized by UV-visible spectroscopy. The optimum experimental conditions for AgNPs synthesis were found to be a temperature of 37oC at pH of 6.0 and a substrate concentration of 2mM silver nitrate after 24 hours incubation times in dark and measured spectrophotometrically at 430 nm.  Silver nanoparticles created were described by different expository procedures, for example, TEM, FT-IR, and X-Ray investigation of both EDX and XRD. The acquired outcomes uncovered that the extent of nanoparticles for all the tried organisms extended from 8.97 to 16.73 nm with variable shapes, a generous portion of them exhibit in a circular nature.


Silver Nanoparticles, Biosynthesis, Optimization, Fungi, Nanobiotechnology.

Cite this article:

Mekawey, A.A.I., Helmy, E.A., 2017. Elucidative Physiological Optimization of Silver Nanospheres Biogenesis by Molds. Int. J. Nanotech. Allied. Sci., 1(1): 30-44.


Ahmad, B., Mukherjee, S., Sastry, M., 2003a. Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum, Colloids Surf. B 28 : 313–318.

Ahmad, A., Senapati, M., Sastry, M., 2003b. Extracellular biosynthesis of monodispersed gold nanoparticles by a novel extermophillic actinomycete, Thermonospora sp., Langmuir 19: 3550–3553.

Akinsiku, A.A., Enock, O.D., Kolawole, O.A., Joseph A.A., Joan, A.A.,  2018. Green Synthesized Optically Active Organically Capped Silver Nanoparticles using Stem Extract of African Cucumber (Momordica charantia).  J. Mater. Environ. Sci., JMES, 9(3): 902-908.

Anil Kumar, S., Abyaneh, M., Ahmad, A., Khan, M.I., 2007. Nitrate reductase mediated synthesis of silver nanoparticles from AgNO3. Biotechnol Lett., 29:439–445.

Banerjee, A., Theron, R., Scott, R.W., 2014. Redispersion of sinthered nanoparticles catalysts in tetra alkylphosphonium ionic liquid. J. Mol. Catal. Chem., 393:105-11.

Bharde, A., Rautaray, D., Bansal, V., Sanyal, M., Sastry, M., 2006. Extracellular biosynthesis of magnetite using fungi. Small  2: 135–141.

Cohen, M.S., Stern, J.M., Libertino, J.A., 2007.  In vitro analysis of a nanocrystalline silvercoated surgical mesh. Surg. Infect. 8: 397–403.

Duran, N., Marcato, P., Esposito, E., 2005. Mechanistic aspects of biosynthesis of silver nanoparticles by several Fusarium oxysporum strains. J. Nanobiotech., 3:8–14.

Elliott, C., 2010.  The effects of silver dressings on chronic and burns wound healing. Br. J. Nurs., 19: 32–36.

Hames, B.O., Rickwood, D. 1990. Gel Electrophoresis of Proteins, a Practical Approach” (2nd Ed.) Oxford  University Press, USA,; pp. 1-147.

Hayat, M.A., 1990. Colloidal Gold: Principles, Methods and Applications, Academic Press, San Diego, CA, 1990.

Jain, N., Bhargava, A., Majumdar, S. and Panwar, 2010. Extracellular biosynthesis and characterization of silver nanoparticles using Aspergillus flavus NJP08: A mechanism perspective. The Royal Society of Chemistry 70-79.

Kathiresan, K., Manivannan, M.A. and Nabeel, B., 2009. Studies on silver nanoparticles synthesized by a marine fungus, Penicillium fellutanum isolated from coastal mangrove sediment. Colloids and Surfaces B: Biointerfaces 71: 133–137.

Kilin, D.S., Prezhdo O.V., Xia, Y., 2008. Shape-controlled synthesis of silver nanoparticles: Ab initio study of preferential surface coordination with citric acid. Chem. Phys. Lett., 6: 113–116.

Kuber, C., Bhainsa, S.F., Souza, D., 2006. Extracellular biosynthesis of silver nanoparticles using the fungus Aspergillus fumigatus Colloids and Surfaces B: Biointerfaces 47: 160–164

Li, Y., Duan, X., Qian, Y., Liao, H., 1999. Nanocrystalline silver particles:  synthesis, agglomeration, and sputtering induced by electron beam. J. Colloid Interface Sci., 209:347-349.

Lowry, O., Rosebrough, A. L., Randall, J., 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem., 193:265-275.

Lu, H.W., Liu, S.H., Jhu, J.K., 2003. Silver nanocrystals by hyperbranched polyurethane-assisted photochemical reduction of  Ag+, Mater. Chem. Phys., 81: 104–107.

Mahendra, R., Alka, Y., Gade, A., 2009. Silver nanoparticles as a new generation of antimicrobials. Biotechnol. Adv., 27: 76–83.

Marcato, P.D., Duran, N., 2008. New aspects of nanopharmaceutical delivery systems. J. Nanosci. Nanotechnol., 8: 2216-2229.

Mohammed, F., Balaji, P., Venkatesanc, R., 2009.  Fungal based synthesis of silver nanoparticles—An effect of temperature on the size of particles. Colloids and Surfaces B: Biointerfaces 74: 123–126.

Nia, P.M., Meng, W.P., Alias, Y., 2015. Hydrogen peroxide sensor: Uniformly decorated silver nanoparticles on polypyrrole for wide detection range. App. Surf. Sci., 357: 1565-72.

Sastry, M., Ahmad, A., Khan, M., 2003. Biosynthesis of metal nanoparticles using fungi and actinomycete. Current Sci., 85:162–170.

Shaligram, N.S., Mahesh, B., Singhal, S., 2009. Biosynthesis of silver nanoparticles using aqueous extract from the compactin producing fungal strain. Process Biochem., 44: 939–943.

Sharmila, C., Ranjith K.R., Chandar, S.B., 2018. Psidium guajava: a novel plant in the synthesis of silver nanoparticles for biomedical applications. Asian J. Pharm. Clin. Res., 11(1): 341-345.

 Singh, R., Singh, N.H., 2011. Medical applications of nanoparticles in biological imaging, cell labeling, antimicrobial agents, and anticancer nanodrugs. J. Biomed. Nanotechnol., 7:489-503.

Solomon, M.M., Umoren, S.A., 2016. In-situ preparation, characterization and anticorrosion property of polypropylene glycol/silver nanoparticles composite for mild steel corrosion in acid solution. J. Colloid Interface Sci., 462:29-41.

Sriram, M. I., Selvaraj, B.K., Kalimuthu, K., 2010. Antitumor activity of silver nanoparticles in Dalton’s lymphoma ascites tumor model. Int. J. Nanomed., 5: 753–762.

Tripathy, A., Ashok, M., Raichur, N., Chandrasekaran,  T., Prathna A., 2010. Process variables in biomimetic synthesis of silver nanoparticles by aqueous extract of Azadirachta indica (Neem) leaves.Nanopart. Res., 12:237–246.

Zhao, G.J., Stevens, S.E., 1998. Multiple parameters for the comprehensive evaluation of the susceptibility of Escherichia coli to the silver ion. Biometals, 11:  27–32.