Abstract:

KPC-producing bacteria are considered one of the most worrisome multidrug-resistant micro-organisms in nosocomial infections. Here we report the draft genomes sequences of two KPC-2 and CTX-M-15 producing Klebsiella pneumoniae sequence type 15 (ST15) and Klebsiella variicola sequence type 32 (ST32) isolates obtained from clinical samples of patients in Rio de Janeiro. A genomic library was constructed using a Nextera XT Kit. An Illumina platform was used to perform whole-genome sequencing (WGS). WGS of isolates K. pneumoniae and K. variicola resulted in estimated genome sizes of 5 662 554 and 5 868 756 bp, respectively. Resistome analysis of the clinical strains revealed the presence of resistance genes to the following antimicrobials in K. pneumoniae: aminoglycosides [aadA1], [aph (3’)-Ia], [aph(3”)-Ib], [aac(6′)-Ib3], [aadA2] and [aph(6)-Id]; beta lactams (bla_OXA-1, bla_oxa-9, bla_CTX-M-15, bla_TEM-1, andbla_SHV-28); carbapenem  (bla_KPC-2); fluoroquinolones [aac(60)-Ib-cr], [oqxA]and [oqxB]; fosfomycin [fosA]; macrolides [mph(A)] and [erm(B)]; phenicols [catB3]; sulfonamides [sul1]; trimethoprim [dfrA12] and tetracycline [tetA] and to K. variicola:  aminoglycosides [aadA1], [aph (3)-IIa], [aac(6′)-Ib-cr] and [aph(6)-Id]; beta lactams (bla_OXA-1,  bla_CTX-M-15, bla_TEM-1B andbla_LEN-2); carbapenem (bla_KPC-2); fluoroquinolones [aac(60)-Ib-cr], [qnrB1], [oqxA]and [oqxB]; fosfomycin [fosA]; phenicols [catB3]; sulfonamides [sul2]; trimethoprim [dfrA14] and tetracycline [tetA]. The resistome revealed by this study might be a useful tool to elucidate the dissemination of nosocomial resistance genes in Rio de Janeiro.

Keywords:

Genome, antibiotic resistance, infection, Klebsiella pneumonia, Klebsiella variicola.

Citation:

Silva, C.R., Miller, R.M., Macedo, V.H.L.M., da Silva, M.A.O., Ronchini, K.R.O.M., Marin, V.A., 2020. Draft Genomes Sequences of KPC-2, OXA-1, OXA-9, SHV-28, TEM-1 and CTX-M-15 Producing Klebsiella pneumoniae ST15 and KPC-2, OXA-1, TEM-1 and LEN-2 Producing Klebsiella variicola ST32 from Clinical Samples in Rio de Janeiro, Brazil. PSM Microbiol., 5(4): 95-101.