Direct Regeneration from Leaf Disc Explants of Peanut: Grafting Improves Survival Rate

Author's: Mahmood ul Hassan, Zahid Akram, Farhat Nazir, Noreen Fatima, Yusuf Zafar, Zaheer Ahmed, Zahid Hussain Shah, Khurram Shahzad, Mahpara Fatima, Madiha Zaynab
Corresponding Author: Mahmood ul Hassan      Email: hmhassanq@hotmail.com
Article Type: Research Article     Published: Jan. 31, 2019 Pages: 7-15
DOI:        Views 709       Downloads0

Abstract:

Leaf discs of 0.5 cm diameter from 10 days old seedlings of four peanut cultivars were cultured on Murashige and Skoog (MS) medium supplemented with twelve different combinations of Thidiazuron (TDZ) and Naphthalene acetic acid (NAA). The highest number of responding explants (52.67%) with the highest number of shoot buds (5.65) per responding explant was achieved at a combination of 0.5 mg/l NAA and 0.5 mg/l TDZ. The explants were then shifted to fresh MS medium containing 8 mg/l Benzylaminopurine (BAP) and 0.5 mg Indole Acetic Acid (IAA) for shoot elongation. Root induction was highest (56.07%) at half-strength MS medium supplemented with 1.5 mg/l NAA. Grafting of in vitro regenerated shoots on 10 days old seedling rootstock showed highest (61.30%) success rate. The survival rate of plants obtained from grafting was almost double (45.76%) than those achieved by rooting of shoots (22.41%). There was significant variation among four varieties regarding the number of responding explant, the number of shoots per explant, rooting or grafting percentage and survival rate. Golden proved to be the best variety in terms of the number of plants reaching maturity.

Keywords:

Grafting, In vitro regeneration, Leaf disc, Naphthalene acetic acid, peanut.

Citation:

Hassan, M., Akram, Z., Nazir, F., Fatima, N., Zafar, Y., Ahmed, Z., Shah, Z.H., Shahzad, K., Fatima, M., Zaynab, M., 2019. Direct Regeneration from Leaf Disc Explants of Peanut: Grafting Improves Survival Rate. Int. J. Nanotechnol. Allied Sci., 3(1): 7-15.

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