Comparative In-vitro Efficacy of Different Acaricides for Controlling Ticks (Acari: Ixodidae) of Veterinary and Public Health Importance

Author's: Amer Ragheb Abdulaziz, Heba S. El-Mahallawy, Abdulaziz M. Almuzaini, Amal A. Hassan
Authors' Affiliations
Article Type: Research Article     Published: Mar. 19, 2019 Pages: 1-12
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Ticks are hematophagous ectoparasites parasitizing farm animals causing great economic losses worldwide, they can also transmit several zoonotic diseases to humans. Tick infestation is a big problem faced by livestock breeders especially the smallholders in Egypt. The efficacy of six commercial acaricides; Cyfluthrin, Deltamethrin, Lambodcyhalothrin, Esbiothrin, Chlorpyrifos, and Malathion were tested in-vitro for controlling the most prevalent tick species in Egypt namely; Boophilus annulatus, Hyalomma dromedarii, and Ripicephalus sanguineus. Ticks were collected from cattle, camels, and dogs, and tested with the targeted acaricides through the larval packet test (LPT) and the disposable pipette method using the recommended concentration and dose by the manufacturer. Resistance level against acaricides was categorized as I, II, III and IV, based on the resistance factor calculated from probit analysis of the obtained data. Cyfluthrin was the most efficacious acaricide against adult and larval stages of all tick species, however, the other five acaricides showed level II of resistance for the larval stage, and level III of resistance for adult ticks (P≤ 0.05). All the used acaricides used showed a better effect on the larval stages than on the adult ticks, and generally the effect was higher with longer exposure time (P≤ 0.05). The obtained data in this study proved the excellent efficacy of Cyfluthrin as an acaricide and pay the attention for its use by the governmental authorities and the veterinarians in their clinics to control Boophilus annulatus, Hyalomma dromedarii, and Ripicephalus sangueneus, in cattle, camels and dogs in Egypt.


Resistance, Acaricides, Cyfluthrin, Deltamethrin, Chlorpyrifos, Malathion, Boophilus annulatus, Hyalomma dromedarii, and Ripicephalus sangueneus.


Abdulaziz, A.R., El-Mahallawy, H.S., Almuzaini, A.M., Hassan, A.A., 2019. Comparative In-vitro Efficacy of Different Acaricides for Controlling Ticks (Acari: Ixodidae) of Veterinary and Public Health Importance. PSM Vet. Res., 4(1): 1-12.


Aboelhadid, S.M., Arafaa, W.M. Mahrousa, L.N., Fahmy, M.M., Kamela, A.A., 2018. Molecular detection of Rhipicephalus (Boophilus) annulatus resistance against deltamethrin in middle Egypt. Vet. Parasitol.: Reg. Stud. Rep., (13): 198-204.

Abdel-Shafy, S., Allam, N.A., Mediannikov, O., Parola, P., Raoult, D., 2012. Molecular detection of spotted fever group rickettsiae associated with ixodid ticks in Egypt. Vector Borne Zoonotic Dis.;12(5): 346-59.

Abdullah, H.H.A.M., El-Shanawany, E.E., Abdel-Shafy, S., Abou-Zeina, H.A.A., Abdel-Rahman, E.H., 2018. Molecular and immunological characterization of Hyalomma dromedarii and Hyalomma excavatum (Acari: Ixodidae) vectors of Q fever in camels. Vet. World., 11(8): 1109-1119.

Adakal, H., Stachurski, F., Chevillon, C., 2013. Tick control practices in Burkina Faso and acaricide resistance survey in Rhipicephalus (Boophilus) geigyi (Acari: Ixodidae), Exp. Appl. Acarol. 59: 483–491,

Barnard, D.R., Jones, B.G., Rogers, G.D., Mount, G.A., 1981. Acaricide susceptibility in the lone star tick: assay techniques and baseline data. J. Econ. Entomol., 74: 466–469.

Coetzer, J.A.W., Tustin, R.C., 2004. Infectious diseases of Livestock (2nd edition). Oxford University Press.

Dantas-Torres, F., 2008. The brown dog tick, Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae): from taxonomy to control. Vet. Parasitol., 152:173–185. DOI: 10.1007/s12639-013-0322-5.

Dutta, S., Godara, R., Katoch, R., Yadav, A., Katoch, M., Singh, N.K., 2017. Detection of amitraz and malathion resistance in field populations of Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) in Jammu region of India. Exp. Appl. Acarol., 71(3): 291-301. doi: 10.1007/s10493-017-0111-4.

Freitas, D. R. J. de, Pohl, P. C., Vaz Junior, I. da S., 2005. Characterization of acaricide resistance in Boophilus microplus. Acta Scientiae Veterinariae. 33(2): 109-117, 2005.

Jyoti, Singh, N.K., Prerna, M., Singh, H., Rath, S.S., 2015. Detection of Malathion Resistance in Hyalomma anatolicum anatolicum from Bathinda District, Punjab. Toxicol. Int.. 22(1): 125-9.

Jyothimol, G., Ravindran, R., Juliet, S., Ajithkumar, K.G., Suresh, N.N., Vimalkumar, M.B., Lenka, D.R., Varghese, S., Ghosh, S., 2014. Low level deltamethrin resistance in ticks from cattle of Kerala, a south Indian state. Vet. Parasitol., 204(3-4):433-438.

Jyoti, Singh, N.K., Singh, H., Rath, S.S., 2016. Multiple mutation in the acetylcholinesterase 3 gene associated with organophosphate resistance in Rhipicephalus (Boophilus) microplus tick from Punjab, India. Vet. Parasitol., 216: 108–117.

Jyoti, Singh, N.K., Singh, H., Rath, S.S., 2014. Malathion resistance in Rhipicephalus (Boophilus) microplus from Ludhiana district, Punjab. J. Parasit. Dis., 38(4):343-346.

Koch, H. G., Burkwhat, H.E., 1984. Susceptibility of the brown dog tick (Acari: Ixodidae) to fresh residues of acaricides: laboratory assays and comparisons of susceptibility of different life stages of the lone star and American dog tick. J. Econ. Entomol., 77: 670–674.

Kumar, R., Nagar, G., Sharma, A.K., Kumar, S., Ray, D.D., Chaudhuri, P., Ghosh, S., 2013. Survey of pyrethroids resistance in Indian isolates of Rhipicephalus (Boophilus) microplus: identification of C190A mutation in the domain II of the para-sodium channel gene. Acta Trop., 125: 237–245.

Kumar, S.S., Rayulu, V.C., Rao, K.S., Kumar, N.V., 2017. Acaricidal resistance in Rhipicephalus (Boophilus) Microplus ticks infesting cattle of Andhra Pradesh. J Entomol. Zool. Stud., 5(6): 580-584.

Mendes, M.C., Lima, C.K., Nogueira, A.H., Yoshihara, E., Chiebao, D.P., Gabriel, F.H., Ueno, T.E., Namindome, A., Klafke, G.M., 2011. Resistance to cypermethrin, deltamethrin and chlorpyriphos in populations of Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) from small farms of the State of Sao Paulo, Brazil. Vet. Parasitol., 178(3-4):383-388.

Miller, R.J., George, J.E., Guerrero, F., Carpenter, L., Welch, J.B., 2001. Characterization of acaricide resistance in Rhipicephalus sanguineus (latreille) (Acari: Ixodidae) collected from the Corozal Army Veterinary Quarantine Center, Panama. J. Med. Entomol., 38:298–302.

Pradeep, B.S., Renukaprasad, C., D’souza, P.E., 2010. Evaluation of the commonly used acaricides against different stages of Rhipicephalus sanguineus by in vitro tests. J. Vet. Parasitol., 24:185-188.

Ram, H., Yadav, C.L., Banerjee, P.S., Kumar, V., 2004. Tick associated mortality in crossbred cattle calves. Indian Vet. J., 81:1203–1205.

Sharma, A.K., Kumar, R., Kumar, S., Nagar, G., Singh, N.K., Rawat, S.S., Ghosh, S., 2012. Deltamethrin and cypermethrin resistance status of Rhipicephalus (Boophilus) microplus collected from six agro-climatic regions of India. Vet. Parasitol., 188(3): 337-345.

Shaw, S.E.,   Day, M.J.,   Birtles, R.J.,  Breitschwerdt, E.B., 2001. Tick-borne infectious diseases of dogs. Trends Parasitol., 17(2): 74-80.

Shyma, K.P., Kumar, S., Sangwan, A.K., Sharma, A.K., Nagar, G., Ray, D., 2013. Acaricide resistance status of Rhipicephalus (Boophilus) microplus and Hyalomma anatolicum collected from Haryana. Indian. J. Anim. Sci., 83(6): 591-594.

Shyma, K.P., Kumar, S., Sharma, A.K., Ray, D.D., Ghosh, S., 2012. Acaricide resistance status in Indian isolates of Hyalomma anatolicum. Exp. Appl. Acarol., 58: 471-481.

Singh, N.K., Gelot, I.S., Jyoti, Singh, V., Rath, S.S., 2015. Detection of amitraz resistance in Rhipicephalus (Boophilus) microplus from North Gujarat, India. J. Parasitic Dis., 39(1): 49-52.

Singh, N.K., Jyoti, Rath, S.S., 2013a. Detection of acaricidal resistance in Hyalomma anatolicum anatolicum. India. Vet. J., 90:17-19.

Singh, N.K., Rath, S.S., 2014. Esterase mediated resistance against synthetic pyrethroids in field populations of Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) in Punjab districts of India. Vet. Parasitol., 204: 330–338.

Soulsby, E.J.L., 1982. Helminths, arthropods and protozoa of domesticated animals, 7th edn. Bailliere Tindal, London, pp 464–465.

SPSS Inc. Released 2007. SPSS for Windows, Version 16.0. Chicago, SPSS Inc.

Sudha, R.R., D’Souza, P.E., Byregowda, S.M., Veeregowda, B.M., Sengupta, P.P., Chandranaik, B.M., Thimmareddy, P.M., 2018. In vitro acaricidal efficacy of deltamethrin, cypermethrin and amitraz against sheep ticks in Karnataka. J. Entomol. Zool. Stud., 6(3): 758-762.

Vatsya, S., Yadav, C.L., 2011. Evaluation of acaricide resistance mechanisms in field populations of Rhipicephalus (Boophilus) microplus collected from India. Int. J. Acarol., 37: 405–410.

Walker, A.R., Bouattour, A., Camicas, J.L., Estrada-Pena, A., Horak, I.G., Latif, A., Pegram, R.G., Preston, P.M., 2003. Ticks of Domestic Animals in Africa. A Guide to Identification of Species. pp 74-221, Bioscience Reports, London, UK.