Can Nanotechnology Improve Detection of the Seed-borne Pathogens through PCR?

Author's: Muhammad Naeem Iqbal, Asfa Ashraf, Azara Iqbal
Corresponding Author: Muhammad Naeem Iqbal      Email:
Article Type: Editorial     Published: Sep. 30, 2019 Pages: Online first
DOI:        Views 19       Downloads0


Pathogens carried on or in seeds which scientifically called seed-borne may cause heavy yield losses (Ali et al., 2017). Late wilt disease caused by Cephalosporium maydis is an important seed-borne disease which transfers through the soil as well as seeds (Michail et al., 1999).

The failure to adequately identify and detect plant pathogens using conventional morphological techniques has led to the development of nucleic acid-based molecular approaches. Immuno-diagnostic tools can also be successfully employed for differential diagnosis, disease surveillance of seed-borne pathogens.

The commonly used seed health tests in laboratories around the world lack specificity, sensitivity, speed, simplicity, cost-effectiveness, and reliability. PCR has many beneficial characteristics that make it highly applicable for detecting pathogens from seeds. In the PCR diagnosis study, the development of DNA extraction is one of the most important steps. These approaches include using AgNPs and FeNPs in the DNA extraction method to enhance the quantity and purity of the DNA template for successful PCR assay.

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Iqbal, M.N, Ashraf, A., Iqbal, A., 2019. Can Nanotechnology Improve Detection of the Seed-borne Pathogens through PCR?. Int. J. Nanotechnol. Allied Sci., 3(3): IJNAS-2019-039.