Bioactivity of Natural Compounds Extracted from Scenedesmus obliquus toward Some Pathogenic Bacteria

Author's: Hanaa H. Morsi¹*, Sabha M. El-Sabbagh¹, Ahlam A. Mehesen², Ahmed Diab Mohamed³
Authors' Affiliations
¹Botany and Microbiology Department, Faculty of Science, Menoufia University, Shebein El- kom, Egypt.²Soil, Water and Environment Research Institute, Algae Unit – Sakha Agriculture Research Station, Sakha, Kafr El-Sheikh, Egypt.³Drinking Water and Sanitation Company, Sidi Salem, Kafr El-Sheikh, Egypt.
*CorrespondenceHanaa H.
Article Type: Research Article     Published: Apr. 30, 2022 Pages: 1-11
DOI:        Views 73       Downloads 0


An assortment of dynamic constituents has been extracted from different types of algae. The expanded utilization of anti-microbials and chemotherapeutics for illness treatment prompts issues of rising of medication safe structures and sway antagonistic impacts on the biological system.  Algae address biomolecules with a wide range of impacts valuable in various biotechnological fields. During this study, various concentrates of the green algae S. obliquus were tried as antibacterial toward three pathogenic bacteria (Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aurues), utilizing BG11 media Scenedesmus obliquus was secluded from freshwater (Albahr elsaeidiu – Mit Yazid – Al-Qadabah) in Kafr El-Sheik governorate. Three solvents (Ethanol, Methanol and Acetone) were utilized for algal extractivity with concentrates of 5, 10, 20 and 40 mg/ml. The antibacterial activity of the concentrates of the algal extracts was determined by agar disc diffusion technique. Antibiotics were utilized to analyze their antibacterial potential. The MIC results were read up for the microorganisms that were vulnerable in the antimicrobial activity test. MIC value of the algal extracts was determined by broth dilution assay technique. Ethanol extract of Scenedesmus obliquus was recorded MICs values (2.25 and 7.12 and 9.5 mg/ml) toward E. coli, P. aeruginosa and S. aureus, individually. GC-MS examination showed that S. obliquus ethanolic extract contain numerous bioactive constituents which are considered with antibacterial impacts, for example, polyphenolic compounds, phenolic compounds, saturated and unsaturated fats, esters, ethers, amide compounds, long chain of hydrocarbons, aldehyde, alcohols and amines-containing compounds.



Solvent extracts,

Pathogenic bacteria,

Antibacterial activity,

Antibiotics, GC-MS.

Authors’ Contribution

SME conceived and designed the study. ADM performed the experiments and analysed data. HHM wrote the paper. AAM edited and revised the paper. HHM finalized the manuscript and gave approval for publication.

How to cite

Morsi, H.H., El-Sabbagh, S.M., Mehesen, A.A., Mohamed, A.D., 2022. Bioactivity of Natural Compounds Extracted from Scenedesmus obliquus toward Some Pathogenic Bacteria. Int. J. Altern. Fuels. Energy., 6(1): 1-11.


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