Bioactivity of Natural Compounds Extracted from Scenedesmus obliquus toward Some Pathogenic Bacteria

Author's: Hanaa H. Morsi¹*, Sabha M. El-Sabbagh¹, Ahlam A. Mehesen², Ahmed Diab Mohamed³
Authors' Affiliations
¹Botany and Microbiology Department, Faculty of Science, Menoufia University, Shebein El- kom, Egypt.²Soil, Water and Environment Research Institute, Algae Unit – Sakha Agriculture Research Station, Sakha, Kafr El-Sheikh, Egypt.³Drinking Water and Sanitation Company, Sidi Salem, Kafr El-Sheikh, Egypt.
*CorrespondenceHanaa H.
Article Type: Research Article     Published: Apr. 30, 2022 Pages: 1-11
DOI:        Views 351       Downloads 0


An assortment of dynamic constituents has been extracted from different types of algae. The expanded utilization of anti-microbials and chemotherapeutics for illness treatment prompts issues of rising of medication safe structures and sway antagonistic impacts on the biological system.  Algae address biomolecules with a wide range of impacts valuable in various biotechnological fields. During this study, various concentrates of the green algae S. obliquus were tried as antibacterial toward three pathogenic bacteria (Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aurues), utilizing BG11 media Scenedesmus obliquus was secluded from freshwater (Albahr elsaeidiu – Mit Yazid – Al-Qadabah) in Kafr El-Sheik governorate. Three solvents (Ethanol, Methanol and Acetone) were utilized for algal extractivity with concentrates of 5, 10, 20 and 40 mg/ml. The antibacterial activity of the concentrates of the algal extracts was determined by agar disc diffusion technique. Antibiotics were utilized to analyze their antibacterial potential. The MIC results were read up for the microorganisms that were vulnerable in the antimicrobial activity test. MIC value of the algal extracts was determined by broth dilution assay technique. Ethanol extract of Scenedesmus obliquus was recorded MICs values (2.25 and 7.12 and 9.5 mg/ml) toward E. coli, P. aeruginosa and S. aureus, individually. GC-MS examination showed that S. obliquus ethanolic extract contain numerous bioactive constituents which are considered with antibacterial impacts, for example, polyphenolic compounds, phenolic compounds, saturated and unsaturated fats, esters, ethers, amide compounds, long chain of hydrocarbons, aldehyde, alcohols and amines-containing compounds.



Solvent extracts,

Pathogenic bacteria,

Antibacterial activity,

Antibiotics, GC-MS.

Authors’ Contribution

SME conceived and designed the study. ADM performed the experiments and analysed data. HHM wrote the paper. AAM edited and revised the paper. HHM finalized the manuscript and gave approval for publication.

How to cite

Morsi, H.H., El-Sabbagh, S.M., Mehesen, A.A., Mohamed, A.D., 2022. Bioactivity of Natural Compounds Extracted from Scenedesmus obliquus toward Some Pathogenic Bacteria. Int. J. Altern. Fuels. Energy., 6(1): 1-11.


Abd El-Hack, M.E., Abdelnour, S.A., Alagawany, M., Abdo, M., Sakr, M.A., Khafaga, A. F., Mahgoub, S.A., Elnesr, S.S., Gebriel, M.G., 2019. Microalgae in modern cancer therapy: Current knowledge. Biomed. Pharmacother., 111: 42-50.

Abedin, R., Taha, H., 2008.  Antibacterial and antifungal activity of cyanobacteria and green microalgae. Evaluation of medium components by Plackett-Burman. Design for antimicrobial activity of Spirulina platensis, Global J. Biotechnol. Biochem., 3: 22-31.

Abubakar, A.R., Haque, M., 2020. Preparation of medicinal plants: basic extraction and fractionation procedures for experimental purposes. J. Pharm. Bioallied Sci., 12: 1-10.

Alishah Aratboni, H., Rafiei, N., Garcia-Granados, R., Alemzadeh, A., Morones-Ramírez, J.R., 2019.Biomass and lipid induction strategies in microalgae for biofuel production and other applications.Microb. Cell Factories, 18: 1-17.

Álvarez-Díaz, P.D., Ruiz, J., Arbib, Z., Barragán, J., Garrido-Pérez, M.C., Perales, J.A., 2015. Wastewater treatment and biodiesel production By Scenedesmus obliquus in a two-stage cultivation process. Bioresour. Technol., 181: 90-96.

Chia, M.A., Lombardi, A.T., Melao, M.D.G.G., 2013. Growth and biochemical composition of Chloralla vulgaris in different growth media. Anais Da Academia Brasileira de Ciências, 85(4): 1427-1438.

Cox, S., Abu-Ghannam, N., 2010. An assessment of the antioxidant and antimicrobial activity of six species of edible Irish seaweeds. Int. Food Res. J., 17(1): 205-220.

Demirel, Z., Yilmaz-Koz, F., Karabay-Yavasoglu, N., Ozdemir, G., Sukatar, A., 2011. Antimicrobial and antioxidant activities of solvent extracts and the essential oil composition of Laurencia obtuse and Laurencia obtusavar. Pyramidata, Rom. Biotech. Lett., 16: 5927-5936.

Desbois, A., Lebl, T., Yan, L., Smith, V., 2009.A fatty acid from the diatom Phaeodactylum tricornutum, as antibacterial toward diverse bacteria including multi-resistant Staphylococcus aureus (MRSA), Marine Biotechnol., 45 – 52.

El-Sheekh, M.M., El Sabagh, S., Abou-El-Souod, G.W., Elbeltagy, A., 2018.The effect of different growth conditions on the biomass and chemical constituents of Chlorella vulgaris. Eg. J. Experim. Biol. (Bot.)., 14(1): 121-131.

El-Sheekh, M.M., Shabaan, M.T., Hassan, L., Morsi, H.H., 2020. Antiviral activity of algae biosynthesized silver and gold nanoparticles toward Herps Simplex (HSV-1) virus in vitro using cell-line culture technique. Int. J. Environ. Health Res., 1-12.

Guedes, A.C., Barbosa, C.R., Amaro, H.M., Pereira, C.I., Malcata, F.X., 2011. Microalgal and cyanobacterial cell extracts for use as natural antibacterial additives toward food pathogens. Int. J. Food Sci. Technol., 46: 862-870.

Ho, S.H., Chen, C.Y., Chang, J.S., 2012. Effect of light intensity and Nitrogen starvation on CO2 fixation and lipid/carbohydrate production of an indigenous microalga Scenedesmus obliquus CNW-N. Bioresour. Technol., 113: 244–252.

Hosseini, N., Akhavan, A., Nowruzi, B., 2019.Detection and Relation of Polyketide Synthase (PKSs) Genes with Antimicrobial Activity in Terrestrial Cyanobacteria of Lavasan. Ir. J. Medical Microbiol., 12(6): 419-31.

Ilavarasi, A., Mubarakali, D., Praveenkumar, R., Baldev, E., Thajuddin, N., 2011.Optimization of various growth media to freshwater microalgae for biomass production. Biotechnol., 10(6): 540-545.

Irith, W., Kai, H., Robert, E., 2008.Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nat. Pro-toc., 3: 163-175.

Jeevanantham, G., Vinoth, M., Hussain, J.M., Muruganantham, P., Ahamed, A.K., 2019. Biochemical characterization of five marine cyanobacteria species for their biotechnological applications. J. Pharmacog. Phytochem., 8(2): 35-40.

Kanimozhi, S., Sridhar, S., 2017. Antioxidant and antibacterial activities of ethyl acetate extract from the thallus of Grateloupia doryphora collected from Kovalum seashore, Tamilnadu. Int. J. Curr. Res., 9: 47846-47852.

Marrez, D.A., Sultan, Y.Y., 2016. Antifungal activity of the cyanobacterium Microcystis aeruginosa toward mycotoxigenic fungi. J. Appl. Pharm. Sci., 6: 191-198.

Marrez, D.A., Sultan, Y.Y., Embaby, M.A., 2017. Biological activity of the cyanobacterium Oscillatoria brevis extracts as a source of nutraceutical and biopreservative agents, Int. J. Pharmacol., 13: 1010-1019.

Nair, B., Krishnika, A., 2011.  Antibacterial activity of freshwater microalga (Scenedesmus sp) toward three bacterial strains, J. Biosci. Res., 2: 160-165.

Najdenski, M., Gigova, G., Iliev, I., 2013. Antibacterial and antifungal activities of selected microalgae and cyanobacteria, Int. J. Food Sci. Technol., 48: 1533-1540.

Okunowo, W.O., Afolabi, L.O., Oyedeji, A.O., Umunnakwea, I.E., Ilesanmi, J.A., 2016. GC-MS analysis and antimicrobial properties of methanolic extracts of the marine algae Skeletonema costatum and Chaetoceros spp. Niger. Soc. Experim. Biol., 28, pp. 24-33.

Ordog, V., Stirk, W., Lenobel, R., Bancírova, M., Strnad, M., Szigeti, J., Nemeth, L., 2014. Screening microalgae for some potentially useful agricultural and pharmaceutical secondary metabolites, J. Appl. Phycol., 16: 309-314.

Pridham, T.G., Lindenfelser, L.A., Shotwell, O.L., Stodola, F.H., Penedict, R.G., Foley, C., Jackson, P.W., Zaumeyer, W .J., Perston, W .H., Miychell, J. W., 1956. Antibiotics toward plant diseases. I. Laboratory and greenhouse survey. Phytopathology, 46, 568-575.

Sahin, S., 2019.Scenedesmus obliquus: a potential natural source for cosmetic industry. Int. J. Second Metab., 6:129-136.

Salem, O., Hoballah, E., Ghazi, S., Hanna, S., 2014. Antimicrobial activity of microalgal extracts with special emphasize on Nostoc sp. Life Sci. J., 11: 752-758.

Tuney, I., Cadirci, B., Unal, D., Sukatar, A., 2006. Antibacterial activities the extracts of marine algae from the coast of Urla (Izmir, Turkey), Turk. J. Biol., 30: 171-175.