Antioxidant and Antibacterial Activities of the species Silene inflata Sm

Author's: Chaima Mouffouk, Soumia Mouffouk, Samia Dekkiche, Leila Hambaba, Sara Mouffouk
Authors' Affiliations
Article Type: Research Article     Published: Apr. 09, 2019 Pages: 74-86
DOI:        Views 781       Downloads0


This study is devoted to the estimation of total phenolic content and the evaluation of in vitro antioxidant and antibacterial activities of crude extracts (petroleum ether, ethyl acetate and methanolic) obtained from the species Silene inflata Sm. The total phenolic content of the obtained extracts was estimated spectrophotometrically by the Folin-Ciocalteu method, the antioxidant activity was determined by scavenging of the free radical DPPH. Furthermore, the antibacterial activity was assessed by agar disk diffusion assay against four bacterial strains. The results of the phytochemical screening of crude extracts revealed the presence of several types of secondary metabolites including steroids, alkaloids, tannins, polyphenols and saponins. The ethyl acetate extract recorded the highest content of polyphenols (8.60 ± 0.01 µg GAE/mg of plant extract) followed by the methanolic and petroleum ether extracts respectively. The results of the antioxidant activity showed that all the tested extracts had the ability to scavenge the free radical DPPH and possess antioxidant activity in a dose-dependent manner. The methanolic extract has a strong antioxidant activity with an IC50 value of 23.53 ± 0.15 μg/ml. The crude extracts (petroleum ether and ethyl acetate) had no antibacterial effect against all the tested strains compared to penicillin and gentamicin as positive controls. While the methanolic extract revealed an antibacterial effect only against the clinical strain Staphylococcus albus with a value of the minimum inhibitory concentration at 0.5 mg/ml. In conclusion, the species Silene inflata Sm. could be an important source of new therapeutic agents against pathological damage due to free radicals and microbial infections.


Silene inflata, phenolic content, phytochemical screening, antioxidant, antibacterial activity.


Mouffouk, C., Mouffouk, S., Dekkiche, S., Hambaba, L., Mouffouk, S., 2019. Antioxidant and Antibacterial Activities of the species Silene inflata Sm. PSM Biol. Res., 4(2): 74-86.


Alarcón, R., García, P., 2006. Caracterización morfológica en poblaciones naturales de colleja (Silene vulgaris). Actas. De. Horticultura., 45: 139-140.

Aldakheel, F.M., Thomas, P.S., Bourke, J.E., Matheson, M.C., Dharmage, S.C., Lowe, A.J., 2016. Relationships between adult asthma and oxidative stress markers and pH in exhaled breath condensate: a systematic review. Allergy., 71(6): 741-757.

Azadi, B., Sohrabi, Y., 2015. Chemical composition of Silene morganae Freyn volatile oil. Nat. Prod. Res., 29(9): 791-794.

Azwanida, N.N., 2015. A review on the extraction methods use in medicinal plants, principle, strength and limitation. Med.  Arom. Plan., 4(196): 2167-0412.

Bajpai, V.K., Shukla, S., Kang, S.C., 2008. Chemical composition and antifungal activity of essential oil and various extract of Silene armeria L. Bioresour. Technol., 99(18): 8903-8908.

Bajpai, V.K., Shukla, S., Kang, S.C., 2008. Chemical composition and antifungal activity of essential oil and various extract of Silene armeria L. Bioresour. Technol., 99(18): 8903-8908.

Balouiri, M., Sadiki, M., Ibnsouda, S.K., 2016. Methods for in vitro evaluating antimicrobial activity: A review. J. Pharma. Anal., 6(2): 71-79.

Bellakhdar, J., 1997. La pharmacopée Marocaine traditionnelle, Ibis Press, France.

Birben, E., Sahiner, U.M., Sackesen, C., Erzurum, S., Kalayci, O., 2012. Oxidative stress and antioxidant defense. World. Allergy. Organ. J., 5(1): 9.

Boğa, M., 2017. Chemical constituents, cytotoxic, antioxidant and cholinesterases inhibitory activities of Silene compacta (fischer) extracts. Marmara Pharm. J., 21(3): 445-454

Ciobanu, C.S., Iconaru, S.L., Le Coustumer, P., Constantin, L.V., Predoi D., 2012. Antibacterial activity of silver-doped hydroxyapatite nanoparticles against gram-positive and gram-negative bacteria. Nanoscale. Res. Lett., 7(1): 324.

Csányi, G., Miller, J.F.J., 2014. Oxidative stress in cardiovascular disease. Int. J. Mol. Sci., 15: 6002-6008.

De Leon, S.P., Wenzel, R.P., 1984. Hospital-acquired bloodstream infections with Staphylococcus epidermidis: review of 100 cases. Am. J. Med., 77(4): 639-644.

Edalatiyan, M.N., Ghahremaninejad, F., Attar, F., Joharchi, M.R., 2011. A taxonomic study on the genus Silene (Caryophyllaceae) in Iran. Rostaniha., 11(2): 133-149.

Ertürk, Ö., Kati, H., Yayli, N., Demirbağ, Z., 2006. Antimicrobial properties of Silene multifida (Adams) Rohrb. plant extracts. Turk. J. Biol., 30(1): 17-21.

Falleh, H., Ksouri, R., Chaieb, K., Karray-Bouraoui, N., Trabelsi, N., Boulaaba, M., Abdelly, C., 2008. Phenolic composition of Cynara cardunculus L. organs, and their biological activities. Comp. Rend. Biol., 331: 372-379.

Fitzpatrick, A.M., Teague W.G., Holguin, F, Yeh, M., Brown, L.A.S., 2009. Program SAR. Airway glutathione homeostasis is altered in children with severe asthma: evidence for oxidant stress. J. Allergy. Clin. Immunol., 123: 146-152.

Fransworth, N.R., 1966. Biological and phytochemical screening of plants. J. Pharm. Sci., 55(3): 225-276

Golea, L., Benkhaled, M., Lavaud, C., Long, C., Haba, H., 2017. Phytochemical components and biological activities of Silene arenarioides Desf. Nat. Prod. Res., 31(23): 2801-2805.

Jorgensen, J.H., Turnidge, J.D., 2015. Susceptibility test methods: dilution and disk diffusion methods. 11nd. ed. In: Manual of clinical microbiology. Am. Soc. Microbiol., 1253-73.

Jürgens, A., 2004. Flower scent composition in diurnal Silene species (Caryophyllaceae): phylogenetic constraints or adaption to flower visitors?. Biochem. Syst. Ecol., 32(10): 841-859.

Jürgens, A., Witt, T., Gottsberger, G., 2002. Flower scent composition in night-flowering Silene species (Caryophyllaceae). Biochem. Syst. Ecol.,  30(5): 383-397.

Karamian, R., Ghasemlou, F., 2013. Screening of total phenol and flavonoid content, antioxidant and antibacterial activities of the methanolic extracts of three Silene species from Iran. Int. J. Agr. Crop. Sci., 5(3): 305-312.

Kerr, S., Brosnan, M.J., McIntyre, M., Reid, J.L., Dominiczak, A.F., Hamilton, C.A., 1999. Superoxide anion production is increased in a model of genetic hypertension. J. Hypertens., 33: 1353-1358.

Li, H.B., Cheng, K.W., Wong, C.C., Fan, K.W., Chen, F., Jiang, Y., 2007. Evaluation of antioxidant capacity and total phenolic content of different fractions of selected microalgae. Food Chem., 102: 771-776.

Mahjoub, S., Masrour-Roudsari, J., 2012. Role of oxidative stress in pathogenesis of metabolic syndrome. Caspian. J. Intern. Med., 3(1): 386-396.

Mamadalieva, N.Z., El-Readi, M.Z., Janibekov, A.A., Tahrani, A., Wink, M., 2011. Phytoecdysteroids of Silene guntensis and their in vitro cytotoxic and antioxidant activity. Z. Naturforschung. C., 66(5-6): 215-224.

Mamadalieva, N.Z., Ul’chenko, N.T., Yuldasheva, N.K., Egamberdieva, D.R., Zhanibekov, A.A., Dzhukharova, M. K., Glushenkova, A.I., 2010. Fatty-acid composition and antibacterial activity of CHCl3 extracts of three plants of the genus Silene. Chem. Nat. Comp., 46(1): 95-96.

Mamadalieva, N.Z., Zibareva, L.N., Saatov, Z., Lafont, R., 2003. Phytoecdysteroids of Silene viridiflora. Chem. Nat. Comp., 39(2): 199-203.

Mégraud, F., 2017. La résistance aux antibiotiques de Helicobacter pylori. Hépato. Gastro. Oncol. Diges., 24(2): 179-181.

Menaceur, F., Hazzit, M., 2014. Comparative study of chemical composition and antioxidant activity of ethanolic extracts from Algerian Lavandula stoechas L. and Rosmarinus tournefortii de Noé. Int. J. Agric. Sci. Res., 4: 39-146.

Montezano, A.C., Dulak-Lis, M., Tsiropoulou, S., Harvey, A., Briones, A.M., Touyz, R.M., 2015. Oxidative stress and human hypertension: vascular mechanisms, biomarkers, and novel therapies. Can. J. Cardiol., 31(5), 631-641.

Morales, P., Carvalho, A.M., Sánchez-Mata, M.C., Cámara, M., Molina, M., Ferreira, I.C., 2012. Tocopherol composition and antioxidant activity of Spanish wild vegetables. Genet. Resour. Crop. Evol., 59(5): 851-863.

Neu, H.C., 1992. The crisis in antibiotic resistance. Science., 257(5073): 1064-1073.

Nicoletti, M., Toniolo, C., Venditti, A., Bruno, M., Ben Jemia, M., 2015. Antioxidant activity and chemical composition of three Tunisian Cistus: Cistus monspeliensis, Cistus villosus and Cistus libanotis. Nat. Prod. Res., 29(3): 223-230.

Rahal, A., Kumar, A., Singh, V., Yadav, B., Tiwari, R., Chakraborty, S., Dhama, K., 2014. Oxidative stress, prooxidants, and antioxidants: The interplay. Biomed. Res. Int., 2014: 7612-7664.

Ray, P.D., Huang, B.W., Tsuji, Y., 2012. Reactive oxygen species (ROS) homeostasis and redox regulation in cellular signaling. Cell. Signal., 24(5): 981-990.

Sayre, L.M., Smith, M.A., Perry, G., 2001. Chemistry and biochemistry of oxidative stress in neurodegenerative disease. Curr. Med. Chem., 8: 721-738.

Sosa, V., Moliné, T., Somoza, R., Paciucci, R., Kondoh, H., LLeonart, M.E., 2013. Oxidative stress and cancer: an overview. Ageing. Res. Rev., 12: 376-390.

Stewart, P.S., Costerton, J.W., 2001. Antibiotic resistance of bacteria in biofilms. The lancet., 358(9276): 135-138.

Tan, J.B.L., Lim, Y.Y., 2015. Critical analysis of current methods for assessing the in vitro antioxidant and antibacterial activity of plant extracts. Food. Chem., 172: 814-822.

Taskin, T., Bitis, L., 2013. Antioxidant activity of Silene alba subsp. divaricata and Stellaria media subsp. media from Caryophyllaceae. Spatula DD., 3(1): 1-5.

Therond, P., 2006. Oxidative stress and damages to biomolecules (lipids, proteins, DNA). Ann. Pharm. Fr., 64: 383-389.

Tramutola, A., Lanzillotta, C., Perluigi, M., Butterfield, D.A., 2017. Oxidative stress, protein modification and Alzheimer disease. Brain. Res. Bul., 133: 88-96.

Witt, T., Jürgens, A., Geyer, R., Gottsberger, G., 1999. Nectar dynamics and sugar composition in flowers of Silene and Saponaria species (Caryophyllaceae).  Plan. Biol., 1(3): 334-345.

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