Antioxidant and Anti-inflammatory Activities of Methanolic Extract of Marrubium deserti de Noé Leaves

Author's: Nabil Ghedadba1, Leila Hambaba1, Messaoud Hachemi1, Mohamed Sabri Bensaad2*
Authors' Affiliations
1Laboratoire de Biotechnologie des Molécules Bioactives et de la Physiopathologie Cellulaire (LBMBPC), Département de Microbiologie et de Biochimie, Faculté des Sciences de la Nature et de la Vie, Université de Batna-2, Batna, Algeria.2Laboratoire de Physio-Toxicologie, Pathologie Cellulaires et Moléculaires-Biomolécules (LPTPCMB), Département de Biologie des Organismes, Faculté des Sciences de la Nature et de la Vie, Université de Batna-2, Batna, Algeria.*CorrespondenceMohamed Sabri BensaadEmail:m.bensaad@univ-batna2.dz
Article Type: Research Article     Published: May. 28, 2021 Pages: 56-65
DOI:        Views 306       Downloads 0

Abstract

The objective of the present study was to determine the pharmacological properties of the methanolic (MeOH) extract of Marrubium deserti leaves. For this purpose, antioxidant activity was carried out by DPPH and Ferric reducing power (FRAP) assays respectivelywhile In vivo anti-inflammatory activity was tested by carrageenan-induced paw edema model. The Phytochemical investigation revealed the presence of several biocompounds, and total phenolic and flavonoid contents were also determined to support our results and revealed a high proportions of polyphenols (184 ± 0.78 mg GAE/g extract) and flavonoids (28.48 ± 0.40 mg QE/g extract). The MeOH extract demonstrated great pharmacological properties with a dose-effect relationship. Thus, a great antioxidant effect was recorded in both DPPH and FRAP assays with a respective IC50 of (15.1 μg/ml) and (80.01 ± 1 µg EAA/g of extract) and were considered significant (P<0.05) when compared to respective standards. On the other hand, anti-inflammatory results suggested that the plant extract could effectively oppose the inflammation caused by carrageenan at the dose of 200 mg/kg with significant decrease (84.1 %) of inflammation. These encouraging results suggest that our plant could be a good candidate to treat more effectively pathologies related to oxidative stress and inflammation.

Keywords

Marrubium deserti de Noé, Antioxidant, Anti-inflammatory. Bioactive compounds.

How to cite

Ghedadba, N., Hambaba, L., Hachemi, M., Bensaad, M.S., 2021. Antioxidant and Anti-inflammatory Activities of Methanolic Extract of Marrubium deserti de Noé Leaves. PSM Biol. Res., 6(3): 56-65.


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