Aflatoxins and Ochratoxin A Content of Stored Yemeni Coffee Beans and Effect of Roasting on Mycotoxin Contamination
Corresponding Author: AbdelRahman A.H. Humaid Email: firstname.lastname@example.org
Article Type: Research Article Published: Apr. 30, 2019 Pages: 11-21
DOI: Views 289 Downloads0
This study was aimed to determine the natural occurrence of ochratoxin A (OTA) and total aflatoxins (B1, B2, G1, and G2) (AFs) of stored Yemeni green and roasted coffee beans. A total of 50 samples (25 samples each) were collected randomly from different markets in Sana’a city, Yemen during 2013, and quantitative assessment of these two types of mycotoxins was done using ELISA test kits. In addition, the influence of roasting on OTA and AFs contamination of green coffee beans was also investigated using the pan roasting method. Our results showed that all green and roasted coffee bean samples examined were contaminated with OTA, at concentrations ranging from 1.114 to 18.667 ppb and 1.646 to 31.077 ppb respectively. Also, all green and roasted coffee bean samples examined were contaminated with AFs, at concentrations ranging from 14.694 to 27.176 ppb and 14.255 to 23.231 ppb respectively. In addition, the roasting of contaminated green coffee bean samples with different concentrations of OTA and AFs reduced OTA and AFs levels to 86% and 20% from the initial concentration, respectively. These results indicate that there are risks of mycotoxin contamination of stored Yemeni green and roasted coffee beans.
Ochratoxin A (OTA), aflatoxins, ELISA kit, green and roasted coffee beans, Yemen.
Humaid, A.A.H., Alghalibi, S.M.S., Al-Khalqi, E.A.A., 2019. Aflatoxins and Ochratoxin A Content of Stored Yemeni Coffee Beans and Effect of Roasting on Mycotoxin Contamination. Int. J. Mol. Microbiol., 2(1): 11-21.
Abu Zinadah, G.M., 2007. Mycotoxins of some Toxigenic Aspergilli and Penicilli Strains on Coffee Seeds in Saudi Arabia. M.Sc. Thesis, Faculty of Sciences, King Abdulaziz University, Saudi Arabia.
Al-Jobory, H.J., Mahmoud, A.L.E., Al-Mahdi, A.Y., 2017. Natural Occurrence of Fusarium Mycotoxins (Fumonisins, Zearalenone and T-2 toxin) in Corn for Human Consumption in Yemen. PSM Microbiol., 2(2): 41-46.
Aydin, G., Ozcelik, N., Cicek, E., Soyoz, M., 2003. Histopathologic Changes in Liver and Renal Tissues Induced by Ochratoxin A and Melatonin in Rats. Human Exp. Toxicol., 22(7): 383-391.
Bandeira, R.D.C.C., Uekane, T.M., Cunha, C.P., Costa, D.A., Cruz, M.H.C., Godoy, R.L.O., Cunha, V.S., 2011. Optimization of Electrospray Terchnique Coupled to Mass Spectrometry for the Determination of Ochratoxin A in Coffee. Rev. de Ci. Vida. Seropédica., 31(2): 45-55.
Bayman, P., Baker, J.L., 2006. Ochratoxins: A Global Perspective. Mycopathogia., 162: 215-223.
Bhat, R., Rai, R.V., Karim, A.A., 2010. Mycotoxins in Food and Feed: Present Status and Future Concerns. Compr. Rev. Food Sci. Food Safety., 9: 57-81.
Bhatnagar, D., Cary, J.W., Ehrlich, K., Yu, J., Cleveland, T.E., 2006. Understanding the Genetics of Regulation of Aflatoxin Production and Aspergillus flavus Development. Mycopathologia., 162: 155–166.
Bilgrami, K.S., 1984. Mycotoxin in Food. J. Ind. Bot. Soc., 63: 976-977.
Blanc, M., Pittet, A., Munoz-Box, R., Viani, R., 1998. Behavior of Ochratoxin A during Green Coffee Roasting and Soluble Coffee Manufacture. J. Agric. Food Chem., 46: 673-675.
Bokhari, F.M., 2007. Mycotoxins and Toxigenic Fungi in Arabic Coffee Beans in Saudi Arabia. Adv. Biol. Res., 1(1-2): 56-66.
Bokhari, F.M., Aly, M.M., 2009a. Trials Towards Reduction of Fungal Growth and Aflatoxin G1 Production in Arabic Coffee Using Different Additives. Afr. J. Food Sci., 3(3): 068-076.
Bokhari, F.M., Aly, M.M., 2009b. Evolution of Traditional Means of Roasting and Mycotoxins Contaminated Coffee Beans in Saudi Arabia. Adv. Biol. Res., 3(3-4): 71-78.
Castellanos-Onorio, O., Gonzalez-Rios, O., Guyot, B., Fontana, T.A., Guiraud, J.P., Schorr-Galindo, S., Durand, N., Suarez-Quiroz, M., 2011. Effect of Two Different Roasting Techniques on the Ochratoxin A (OTA) Reduction in Coffee Beans (Coffeaarabica). Food Control., 22: 1184-1188.
Chu, F.S., 1997. Mode of Action of Mycotoxins and Related Compounds. Adv. Appl. Microbiol., 40: 352-357.
COE. (Council of Europe Publishing Editions du Counseil de l-Europe), 2008. Guidelines for Risk Assessment, Prevention and Management: Health Risks and Safety Hazards Related to Pest Organisms in Stored Products. Council Europe Publishing, pp. 90.
Copetti, M.V., Iamanaka, B.T., Pereira, J.L., Lemes, D.P., Nakano, F., 2012. Co-occurance of Ochratoxin A and Aflatoxins in Chocolate Marketed in Brazil. Food Control., 26: 36-41.
Diaz, G.J., Ariza, D., Perilla, N.S., 2004. Method Validation for the Determination of Ochratoxin A in Green and Soluble Coffee by Immunoaffinity Column Cleanup and Liquid Chromatography. Mycotoxin Res., 20: 59-67.
- (European Commission), 2002. Task 3.2.7 “Assessment of Dietary Intake of Ochratoxin A by the Population of EU Member States”. Rome, Italy, January.
- (European Commission), 2006. Regulation (EC) 1881/2006 of 19/12/2006. Official J. Eu. Commun., L 364, pp. 5-24.
FAO. (Food and Agricultural Organization), 2006. Reducing Ochratoxin A in Coffee. Available at: http://www.coffee-ota.org/frq.asp.
Farah, A., 2012. Coffee Constituents. In: Coffee: Emerging Health Effects and Disease Prevention, First edition. Ed. Chu, Y.-F., Blackwell Publishing Ltd., pp. 21-58.
Gollücke, A.P.B., Taniwaki, M.H., Tavares, D.Q., 2004. Survey on Ochratoxin A in Brazilian Green Coffee Destined for Exports. Ciência e Tecnologia de Alimentos, Campinas, 24(4): 641-645.
Heilmann, W., Rehfeldt, A.G., Rotzoll, F., 1999. Behaviour and Reduction Ochratoxin A in Green Coffee Beans in Response to Various Processing Methods. Eur. Food Res. Technol., 209: 297-300.
Hill, R.A., Wilson, D.M., McMillan, W.W., Widstrom, N.W., Cole, R.J., Sanders, T.H., Andblankenskip, P.D., 1985. Ecology of the Aspergillusflavus Group and Aflatoxin Formation in Maize and Groundnut. In: Trichothecenes and Other Mycotoxins. Ed. Lacey, J., Chichester., John Wiley, pp. 79- 95.
IARC. (International Agency for Research on Cancer), 1993. Ochratoxin A. In: IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, some Naturally Occurring Substances: Food Items and Constituents, Heterocyclic Aromatic Amines and Mycotoxins, International Agency for Research on Cancer: Lyon, France, 56: 489-571.
IARC. (International Agency for Research on Cancer), 2002. Aflatoxins. In: IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, some Traditional Herbal Medicines, some Mycotoxins, Naphthalene and Styrene, International Agency for Research on Cancer: Lyon, France, 82: 171-366.
Iqbal, M.N, Ashraf, A., Ling, S., Wang, S., 2018. In Vitro Improved Production of Monoclonal Antibody against Zearalenone in Supplemented Cell Culture Media. PSM Biol. Res., 3(3): 106-110.
JECFA. (Joint Expert Committee on Food Additive and Contaminants), 1995. Evaluation of Certain Food Additive and Contaminants. In: Technical Report Series n. 859, Geneva: Joint FAO/WHO Expert Committee on Food Additives.
Jorgensen, K., 1998. Survey of pork, poultry, coffree, bear and pulses for ochratoxin A. Food Addit. Contam., 15: 550.
Kaushik, A., Arya, S.K., Vasudev, A., Bhansali, S., 2013. Recent Advances in Detection of Ochratoxin-A. Open J. Appl. Biosensor., 2: 1-11.
Kummer, C., 2003. The Joy of Coffee: The Essential Guide to Buying, Brewing, and Enjoying. Houghton Mifflin, Boston, pp. 37.
Leoni, L.A.B., Soares, L.M.V., Liveira, L.P.C., 2000. Ochratoxin A in Brazilian Roasted and Instant Coffees. Food Addit. Contam., 17: 867-870.
Maaroufi, K., Pfohl-Lesszkowicz, A., Achour, A., May, M., Grosse, Y., Hammami, M., Ellouz, F., Creppy, E.E., Bacha, H., Nchartoxin, A., 1994. Ochratoxin A Genotoxicity, Relation to Renal Tumors. Arch. de La Institute Pasteur de Tunis., 71(1-2): 21-31.
Machado, E.M.S., Rodriguez-Jasso, R.M., Teixeira J.A., Mussatto, S.I., 2012. Growth of Fungal Strains on Coffee Industry Residueswith Removal of Polyphenolic Compounds. Biochem. Engin. J., 60: 87-90.
Mahmoud, A.L.E., 1993. Toxigenic Fungi and Mycotoxic Content in Poultry Feed stuff Ingredients. J. Appl. Microbiol., 84: 62-76.
Nakajima, M., Tsubouchi, H., Miyabe, M., Ueno, V., 1997. Survey of Aflatoxin B1 and Ochratoxin A in Commercial Green Coffee Beans by HPLC Linked with Immunoaffinity Chromatography. Food Agric. Immunol., 9(2): 77-83.
Nasser, L.A., 2008. Mycoflora, Mycotoxins, Bacteriological Analysis and Molecular Assay of some Bacterial Species from Coffee Beans inSaudi Arabia. Bulletin of Pharmaceutical Sciences, Assiut University, 31: 345-373.
Nehad, E.A., Farag, M.M., Kawther, M.S., Abdel-Samad, A.K.M., Naguib, K., 2005. Stability of Ochratoxin A (OTA) during Processing and Decaffeination in Commercial Roasted Coffee Beans. Food Additives and Contaminants, 22: 761-767. In cited: Makun, H.A. (2013): Mycotoxin and Food Safety in Developing Countries. INTECH Publishing, pp. 182.
Nogaim, Q.A., Gowri, P.M., 2013. Determination of Ochratoxin A in Yemeni Green Coffee. Scholars Acad. J. Biosci., 1(6): 253-262.
Ogunsanwo, B.M., Faboya, O.O.P., Idowu, O.R., Lawal, O.S., Bankole, S.A., 2004. Effect of Roasting on the Aflatoxin Contents of Nigerian Peanut Seeds. Afr. J. Biotechnol., 3(9): 451-455.
Ozer, H., OktayBasegmez, H.I., Ozay, G., 2012. Mycotoxin Risks and Toxigenic Fungi in Date, Prune and Dried Apricot among Mediterranean Crops. Phytopathol. Mediterr., 51(1): 148-157.
Pardo, E., Marin, S., Ramos, A.J., Sanchis, V., 2004. Occurrence of Ochratoxigenic Fungi and Ochratoxin A in Green Coffee from Different Origins. Food Sci. Technol. Int., 10: 45-49.
Pariza, W.M., 1996. Toxic Substances. In: Food Chemistry. Ed. Fennema, O. R., 3rd Edition, Marcel Dekker, New York, pp. 825-841.
Perrone, D., Donangelo, C.M., Farah, A., 2008. Fast Simultaneous Analysis of Caffeine, Trigonelline, Nicotinic Acid and Sucrose in Coffee by Liquid Chromatography-Mass Spectrometry. Food Chem., 110: 1030-1035.
Romani, S., Sacchetti, G., Chaves Lopez, C., Pinnavaia, G.G., Dalla Rosa, M., 2000. Screening on the Occurrence of Ochratoxin A in Green Coffee Beans of Different Origins and Types. J. Agric. Food Chem., 48: 3616-3619.
Romani, S., Pinnavaia, G.G., Roas, M.D., 2003. Influence of Roasting Levels on Ochratoxin A Content in Coffee. J. Agric. Food Chem., 51: 5168-5171.
Salem, N.M., Ahmed, R., 2010. Mycotoxins in Food from Jordan: Preliminary Survey. Food Control., 21: 1099-1103.
Shinha, K.K., Bhatnagar, D., 1998. Mycotoxins in Agriculture and Food Safety. CRC Press, pp. 387.
Soliman, K.M., 2002. Incidence, Level and Behavior of Aflatoxins during Coffee Bean Roasting and Decaffeination. J. Agric. Food Chem., 50(25): 7477-7481.
Studer-Rohr, I., Dietrich, D.R., Schlatter, J., Schlatter, C., 1994. Ochratoxin A and Coffee. MitteiLungenAus Dem Gebiete Der Lebensmittelunt. Ersuchung and Hyg., 85: 719-727.
Suárez-Quiroz, M., González-Rios, O., Barel, M., Guyot, B., Schorr-Galindo, S., Guiraud, J., 2004. Study of Ochratoxin A-Producing Strains in Coffee Processing. Int. J. Food Sci. Technol., 39: 501-507.
Tsubouchi, H., Yamamoto, K., Hisada, K., Sakabe, Y., Udagawa, S., 1987. Effect of Roasting on Ochratoxin A Level in Green Coffee Beans Inoculated with Aspergillus ochraceus. Mycopathologia., 97(2): 111-115.
USAID. (United States Agency for International Development), 2005. Moving Yemen Coffee Forward. Assessment of the Coffee Industry in Yemen to Sustainably Improve Incomes and Expand Trad. This Publication Was Produced for Review by United State Agency for International Development, It was Prepared by ARD, Inc. December, 2005.
Van der Stegen, G.H., Essens, P.J., Van der Lijn, J., 2000. Effects of Roasting Conditions on Reduction of Ochratoxin A in Coffe. J. Agric. Food Chem., 49(10): 473- 475.
Van der Stegen, G.H.D., Essens, P.J.M., Van der Lijn, J., 2001. Effects of Roasting Conditions on Reduction of Ochratoxin A in Coffee. Journal of Agricultural and Food Chemistry, 49: 4713-4715. In cited: Makun, H.A. (2013): Mycotoxin and Food Safety in Developing Countries. INTECH Publishing, pp. 182.
Vanesa, D., Ana, P., 2013. Occurance of Ochratoxin A in Coffee Beans, Ground Roasted Coffee and Soluble Coffee and Method Validation. Food Control., 30: 675-678.
Vasanthi, S., Baht, R.V., 2000. Ochratoxin in Indian Coffee and Its Implication to International Trade. J. Nutr., 21: 718-721.
Viane, R., 2002. Effect of Processing on Ochratoxin A (OTA) Content of Coffee. In: Mycoyoxins and Food Safety. Eds. Devries, J.W., Truchsess, M.W. and Jackson, L.S, Springer, vol. 504, pp. 189-193.
Weidenbörner, M., 2001. Encyclopedia of Food Mycotoxins. Springer Science & Business Media, pp. 15.
Whitaker, T.B., Slate, A.B., 2006. Sampling Almonds for Aflatoxin, Part I: Estimation of Uncertainty Associated with Sampling, Sample Preparation, and Analysis. J. AOAC Int., 89(4): 1027-1034.
Zaied, S.A.F., Aly, S.E., EL-Sideek, L.E., Alkhalifah, D.H.M., 2012. Electron Spin Resonance (ESR) Studies on-Irradiated and Roasting Coffee Beans during Decontaminated of OTA. Austr. J. Basic Appl. Sci., 6(3): 741-750.
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