The present study was planned to evaluate the transgenic potato plants containing chitinase (RCG-3) gene transformed through Agrobacterium for resistance against Rhizoctonia Solani and Fusarium solani. In vitro, plants were cultured for multiplication and then pathogenicity of transgenic potato plants harboring chitinase gene were carried out with fungal pathogens (R. solani and F. solani). The fungal pathogens were isolated from infected tubers and purified by single spore technique. For multiplication of pathogens wheat straw method was used for R.solani and sorghum seed method was used for F.solani. The inoculation of fungal infection (R. solani) and (F. solani) transgenic and non-transgenic plants were performed in laboratory and controlled greenhouse conditions (Containment). Results showed that transgenic potato plants containing chitinase (RCG-3) gene transformed through Agrobacterium showed resistance against R. Solani and F. solani compared to non-transgenic plants. It was concluded that rice chitinase gene expression in potato confers enhanced resistance against two major fungal diseases of potato in Pakistan.
Keywords: Dry rot, Fusarium wilt, Tissue culture, Transgenic, Vector .
Cite this article: Zaynab, M., Kanwal, S., Hussain, I., Qasim, M., Noman, A., Iqbal, U., Ali, G.M., Bahadar, K., Jamil, A., Sughra, K., Rehman, N., Buriro, M., Abbas, S., Ali, M., Alvi, A.H., Anwar, M., Khan, M.I., Tayyab, M., 2017. Rice Chitinase Gene Expression in Genetically Engineered Potato Confers Resistance against Fusarium solani and Rhizictonia solani. PSM Microbiol., 2(3): 63-73.Full Text