Four brands of Amoxicillin trihydrate (Alomox LA, Amovet LA, Farmox LA and Novamox LA) were processed for chemical equivalence analysis by high performance liquid chromatography (HPLC) and their minimum inhibitory concentrations (MICs) against Staphylococcus aureus and Escherichia coli by micro-dilution method. The content of amoxicillin in each brand was found acceptable by HPLC. The highest content (101.22%) was found in Farmox LA and the lowest content (90.72%) in Amovet LA. The MIC of amoxicillin against S. aureus and E. coli was 0.25µg/mL and 8µg/mL, respectively. E. coli (26.67%) and S. aureus (13.33%) had moderate resistance to amoxicillin. It was concluded that quality of all brands was acceptable and can be used for E. coli and S. aureus infections due to moderate resistance.
Keywords:Amoxicillin trihydrate, chemical equivalence, HPLC, E. coli, S. aureus and minimum inhibitory concentration.
To cite this article: Ali, R.A., Ashraf, M., Rasheed, M.A., Anjum, A.A., Rathore, A.W., Khan, A.B, Ali, M.A., Iqbal, A., 2016. Chemical Equivalence of Different Brands of Amoxicillin Trihydrate and Its Efficacy against Bacterial Isolates. PSM Microbiol., 01(1): 45-49.