Determination of Histamine in Various Marine Fish Species Available in Major Markets of Punjab, Pakistan by LC-MS/MS


Fish samples from selected five (5) major markets of Punjab (Lahore, Faisalabad, Multan, Rawalpindi and Gujranwala) were collected randomly consisting of nine (9) marine fish species. LCQ Fleet with ion trap mass analyzer coupled with Surveyorplus pump and Surveyorplus degassor of Thermoscientific was used for histamine determination in present study. The histamine detected in different nine (9) samples from different markets was within safe limits. The fishes were also examined for appearance, texture, odour and presence of black spots. All the physical parameters were within normal range. It was concluded that the marine fish species being sold in various fish markets of Punjab province are fresh with good physical conditions and having histamine in safe limits.


Histamine, Marine Fish, Punjab, Pakistan, LCMS, Markets, FQCL.


Hadyait, M.I., Ali, A., Bhatti, E.M., Qayyum, A., Ullah, M.Z., 2018. Determination of Histamine in Various Marine Fish Species Available in Major Markets of Punjab, Pakistan by LC-MS/MS. PSM Biol. Res., 3(1): 39-43.

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Association of Official Analytical Chemists., 2012. Histamine in Seafood. AOAC, 35, 15-19.

Ali. A., Waheed. K.N., Hadyait. A., Begum. I., Hayat. S., 2016. Determination of histamine levels by LC-MS/MS in various fish species available in the local markets of Punjab, Pakistan. IJFAS, 4(6): 128-132.

Becker. K., Southwick. K., Reardon. J., Berg. R., Maccormack. J.N., 2001. Histamine poisoning associated with eating tuna burgers. JAMA, 285: 1327-1330.

Behling, A., Taylor. S., 1982. Bacterial histamine production as a function of temperature and time of incubation. J. Food Sci., 47: 1311-1317.

Chamberlain, T., 2001. Histamine levels in longlined tuna in Fiji: a comparison of samples from two different body sites and the effect of storage at different temperatures. S. Pac. J. Nat. Sci., 19: 30-40.

Eitenmiller, R., Desouza, S., 1984. Enzymatic Mechanisms for amine formation in Fish and in seafood toxins. (Edited by E.P. Ragelis). American chemical Society, Washington, D.C., 443-442.

FDA., 2013. ORA Laboratory manual. Document no. version, 4(09): 1-5.

Hall, M., 2003. Something fishy: six patients with an unusual cause of food poisoning. Emerg. Med. (Fremantle), 15(3): 293-295.

Joshi, P. A., Vishal, B.S., 2011. Study of Histamine Forming Bacteria in Commercial Fish Samples of Kalyan City. Int. J. Curr. Sci. Res., 1(2): 39-42.

Lehane, L., Olley, J., 2000. Histamine fish poisoning revisited. Int. J. Food Microbiol., 58(1-2): 1-37.

Moreno, R. B., Torres, E.A., 2001. Histamine levels in fresh fish, a quality index. Session 42, Seafood Technology, Safety, Processing, IFT Annual Meeting. New Orleans, Louisiana.

Nahla, T.K., Hassan, E. L. S., Farag, M., 2005. Histamine and Histamine Producing Bacteria in Some Local and Imported Fish and Their Public Health Significance. Res. J. Agric. Biol. Sci., 1(4): 329-336.

Sabroe. R., Kobza. B., 1998. Scombrotoxic Fish Poisoning. Clin. Exp. Dermatol., 23(6): 258-259.

Author: Muhammad Arfan Hadyait, Asif Ali, Ehsan Mahmood Bhatti, Aroosa Qayyum, Muhammad Zafar Ullah

Pages: 39-43

Published Online: Jan. 31, 2018

Article Type: Research Article

Views: 172

Downloads: 0

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